不同禾本科作物中ZmWRKY79同源基因的鉴定与分析  

Identification and analysis of ZmWRKY79 homologous genes in different gramineal crops

作　　者：卢源达 陈玲[2] 杜云龙[1] 刘学思 柯学[2] 赵建屛 吴洪钦 刘鑫 李玉琢 朱文强 邢世军 程在全[2] 钟巧芳[2] LU Yuan-da;CHEN Ling;DU Yun-long;LIU Xue-si;KE Xue;ZHAO Jian-ping;WU Hong-qin;LIU Xin;LI Yu-zhuo;ZHU Wen-qiang;XING Shi-jun;CHENG Zai-quan;ZHONG Qiao-fang(College of Plant Protection,Yunnan Agricultural University,Kunming 650224,China;Institute of Biotechnology and Germplasm Re-sources,Yunnan Academy of Agricultural Sciences/Key Laboratory of Agricultural Biotechnology,Yunnan Province/Key Laboratory for Gene Resources and Germplasm Creation of Crops in Southwest China,Ministry of Agriculture,Kunming 650205,China)

机构地区：[1]云南农业大学植物保护学院,昆明650224 [2]云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创制重点实验室,昆明650205

出　　处：《西南农业学报》2023年第3期522-531,共10页Southwest China Journal of Agricultural Sciences

基　　金：云南省颜龙安院士专家工作站(202005AF150032);云南省基础研究专项面上项目(202001AT070015、202001AT070003);云南省科技人才和平台计划项目(2019HB034、202005AM070029)。

摘　　要：【目的】玉米ZmWRKY79基因在合成植保素和干旱胁迫响应中发挥重要作用。文章旨在通过生物信息学技术从不同禾本科作物中挖掘更多的ZmWRKY79同源基因,为玉米及其它禾本科作物提供抗逆境胁迫的候选基因。【方法】从NCBI数据库中获取玉米ZmWRKY79同源基因的基本信息,并对其进行染色体定位、基因结构可视化、功能结构域预测、进化关系分析、亚细胞定位预测、启动子顺式作用元件预测及非生物胁迫表达预测。【结果】在玉米、谷子、高粱、青稞、野生二粒小麦、普通小麦、水稻作物中共鉴定到9个ZmWRKY79同源基因,这些基因结构相似性较高,分布于不同的染色体上,且均属于第III类WRKY转录因子。系统进化分析结果显示ZmWRKY79蛋白同源序列可被分为I和II类,不同成员间进化受纯化选择,其中ZmWRKY79蛋白与高粱SbWRKY54蛋白同源性较高,达到81.58%。亚细胞定位预测显示所有ZmWRKY79同源蛋白均在细胞核,说明ZmWRKY79同源基因均在细胞核中发挥功能。启动子顺式作用元件预测显示ZmWRKY79同源基因的启动中主要包含组织特异性元件、应激响应元件和激素响应元件。非生物胁迫表达预测表明,ZmWRKY53和ZmWRKY74 2个ZmWRKY79的同源基因被确定为受逆境胁迫诱导。【结论】在7种禾本科作物中鉴定到9个ZmWRKY79同源基因,这些同源基因可能具有抗逆功能,为禾本科作物抗逆研究提供了更多的基因选择。【Objective】MaizeZmWRKY79gene plays an important role in the synthesis of phytoalexins and drought stress response.It is meaningful to mine moreZmWRKY79homologous genes in different gramineous crops through bioinformatics technology,and provide candi⁃date genes for stress resistance in maize and other gramineous crops.【Method】In the study,the information of maizeZmWRKY79homologous genes was retrived from NCBI database.These genes were subjected to bioinformatical analyses,including chromosome localization,gene structure visualization,functional domain prediction,evolutionary relationship analysis,subcellular localization prediction,promoter cis⁃act⁃ing element prediction and abiotic stress expression prediction.【Result】A total of nineZmWRKY79homologs were identified in maize,grain,sorghum,barley,wild two⁃grain wheat,common wheat,and rice crops,which were structurally similar,distributed in different chro⁃mosomes and belonged to the class III WRKY transcription factors.The homologous sequences of ZmWRKY79 protein could be categorized into classes I and II based on phylogenetic analysis,and the evolution between distinct members was susceptible to purifying selection.ZmWRKY79 protein shared a high similarity(81.58%)with sorghum SbWRKY54 protein.The predicted results of subcellular lo⁃calization showed that all ZmWRKY79 homologous proteins were in the nucleus,indicating that allZmWRKY79homologous genes functioned in the nucleus.The prediction of promoter cis⁃acting elements showed that theZmWRKY79homologous gene contained mainly tissue⁃specific,stress⁃responsive and hormone⁃responsive elements in its initiation.Two homologs ofZmWRKY79,ZmWRKY53andZmWRKY74were predic⁃ted to be induced by abiotic stress based on a prediction of their expression in response to adversity.【Conclusion】NineZmWRKY79homologs were identified in 7 graminaceous crops,which might have resistance functions and provide additional genetic options for graminaceous crop resistance studies.

关 键 词：玉米 ZmWRKY79 同源基因 生物信息学 

分 类 号：S513[农业科学—作物学]