基于隶属函数和聚类分析法的广西韭菜地方种质资源耐热性评价  

Evaluation of heat tolerance of leek local germplasm resources in Guangxi based on membership function and cluster analysis

作　　者：王萌[1] 赵曾菁 赵虎[1] 吴星[1] 龚明霞[1] 何志[1] 王日升[1] WANG Meng;ZHAO Zeng-jing;ZHAO Hu;WU Xing;GONG Ming-xia;HE Zhi;WANG Ri-sheng(Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China)

机构地区：[1]广西农业科学院蔬菜研究所,南宁530007

出　　处：《西南农业学报》2023年第3期541-549,共9页Southwest China Journal of Agricultural Sciences

基　　金：广西创新驱动发展专项(桂科AA17204045);广西农业科学院科技发展基金项目(桂农科2021JM94)。

摘　　要：【目的】建立韭菜种质资源耐热性评价方法,为选育耐热韭菜品种及丰富广西耐热韭菜品种资源提供参考依据。【方法】以农业农村部第三次种质资源普查与收集行动(2015—2020)期间收集的广西大叶韭、宽叶韭和细叶韭3类韭菜地方种质资源(分别标记为A、B、C、D、E、F、G、H和I)为试验材料,测定其38℃高温胁迫(实验室组)处理7 d后的生长指标(叶片最大生长长度和地上部新增生物量)和生理指标[叶绿素、脯氨酸(Pro)和丙二醛(MDA)含量],结合田间自然高温胁迫(田间组)实际耐热表现,利用多元统计分析法(主成分分析、隶属函数法和聚类分析法)综合评价不同类型韭菜种质资源的耐热性。【结果】在38℃高温胁迫下,广西大叶韭、宽叶韭和细叶韭3类9份韭菜种质的叶片最大生长长度和地上部新增生物量均较其适温对照(CK)极显著下降(P<0.01),其中,降幅最大的是大叶韭类型,降幅最小的是细叶韭类型;叶绿素含量均有不同程度下降,其中,降幅最大的是大叶韭类型,降幅最小的是细叶韭类型;MDA含量均有不同程度上升,其中,升幅最大的是大叶韭类型,升幅最小的是细叶韭类型;Pro含量均有不同程度上升,其中,升幅最大的是细叶韭类型,升幅最小的是大叶韭类型。综合评价结果表明,广西3类韭菜地方种质资源的耐热性排序为细叶韭>宽叶韭>大叶韭,9份韭菜种质中耐热性较强的是种质G(No.2016451159)和种质I(No.2016453604),耐热性最弱的是种质C(No.SC20191200)。【结论】以叶片最大生长长度、地上部新增生物量及叶绿素、Pro和MDA含量作为韭菜种质资源耐热性评价指标,采用主成分分析法、隶属函数法和聚类分析法可将广西3类韭菜种质资源的耐热性排序为细叶韭>宽叶韭>大叶韭,并确定种质G和I为耐热性较强种质,种质C为耐热性最弱种质。【Objective】The present paper aimed to establish the evaluation method of heat resistance of leek resources,select heat⁃resistant leek varieties,and solve the problem of shortage of heat⁃resistant leek varieties in Guangxi.【Method】Taking three kinds of local germplasm resources of leek(marked as A,B,C,D,E,F,G,H and I,respectively)of Guangxi large leaf leek,wide leaf leek and thin leaf leek col⁃lected from the general survey of germplasm resources by Ministry of Agricultural and Rural Affairs(2015-2020)as test materials,the changes of the maximum growth length of leek leaves,the newly increased aboveground biomass and the contents of chlorophyll,proline and malondialdehyde after 7 days of high temperature stress at 38℃were measured.Combined with field natural high temperature stress(field group)actual heat resistance performance,the heat resistance of different types of leek was comprehensively evaluated by multivariate statis⁃tical analysis(variance analysis,principal component analysis,membership function method and cluster analysis)methods.【Result】Under high temperature stress of 38℃,the maximum leaf growth length and the newly increased biomass of 9 leek germplasms of the three types in Guangxi were extremely significantly decreased compared with their proper temperature control(CK)(P<0.01).The largest decline was the type of large⁃leaved leek,and the smallest decline was the type of fine⁃leaved leek.Chlorophyll content decreased to different degrees,of which the largest decline was the type of leek with large leaves,and the smallest decline was the type of leek with thin leaves.The content of MDA increased to different degrees,of which the largest increase was the type of leek with large leaves,and the smallest increase was the type of leek with thin leaves.Pro content increased to different degrees,of which,the largest increase was the type of leek with thin leaves,and the smallest increase was the type of leek with large leaves.The comprehensive evaluation results showed that the

关 键 词：韭菜 种质资源 高温胁迫 耐热性评价 广西 

分 类 号：S633.3[农业科学—蔬菜学]