四川抗赤霉病小麦品种(系)抗病基因检测  

Molecular detection of resistance genes in Sichuan Fusarium head blight(FHB)resistant wheat varieties(lines)

作　　者：邓清燕 罗江陶[1,2,3] 郑建敏 万洪深[1,2,3] 李式昭 杨漫宇[1,2,3] 夏先全 蒲宗君[1,2,3] DENG Qing-yan;LUO Jiang-tao;ZHENG Jian-min;WAN Hong-shen;LI Shi-zhao;YANG Man-yu;XIA Xian-quan;PU Zong-jun(Crop Research Institute,Sichuan Academy of Agricultural Science,Chengdu 610066,China;Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China(Ministry of Agriculture and Rural Affairs of the People’s Republic of China),Chengdu 610066,China;Environment-friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province,Chengdu 610066,China;Institute of Plant Protection,Sichuan Academy of Agricultural Science,Chengdu 610066,China)

机构地区：[1]四川省农业科学院作物研究所,成都610066 [2]农业农村部西南地区小麦生物学与遗传育种重点实验室,成都610066 [3]粮油作物绿色种质创新与遗传改良四川省重点实验室,成都610066 [4]四川省农业科学院植物保护研究所,成都610066

出　　处：《西南农业学报》2023年第3期575-583,共9页Southwest China Journal of Agricultural Sciences

基　　金：四川省财政专项(2021ZYGG-003);四川省十四五麦类育种攻关项目(2021YFYZ0002);四川省农业科学院现代学科建设推进工程项目(2021XKJS005)。

摘　　要：【目的】旨在研究四川小麦抗赤霉病的基因资源,培育赤霉病综合抗性中抗以上的小麦品种。【方法】利用土表接种法鉴定出156份赤霉病达中抗水平的小麦品种或高代品系,通过已知赤霉病抗性基因Fhb1、Fhb2、Fhb4和Fhb5以及部分抗条锈病基因和抗白粉病基因的分子标记检测抗性基因的组成。【结果】经抗病基因分子标记检测,在供试的156份赤霉病抗性材料中仅检测出2份材料(20引979和20间2506-10)分别含有抗赤霉病基因Fhb1和Fhb5。同时,检测出含小麦条锈病抗性基因Yr5、Yr15、Yr18、Yr26和YrAs2388的材料分别为4、63、9、3、14份,在供试材料中的分布频率分别为2.56%、40.38%、5.77%、1.92%和8.97%。检测出含白粉病抗性基因Pm21的材料44份,在供试材料中的分布频率为28.21%。抗病基因的分布表明,122份供试材料含有至少1个抗病基因,其中6份材料含有条锈病和白粉病2种抗病基因,9份材料中聚合了2个抗条锈病基因,剩余的106份材料仅含有1个抗病基因。仅1份供试材料(20间2506-10)同时含有赤霉病、条锈病和白粉病3种抗病基因。【结论】四川小麦缺乏遗传背景明确的抗赤霉病资源,因此四川小麦抗赤霉病育种在充分利用本地抗赤霉病资源的同时应加强引进已知抗源的资源加以利用。【Objective】In recent years,the wheat in Sichuan province is threatened byFusariumhead blight(FHB)or scab as changes in climate and farming practices.The present paper aimed to obtain the genetic resources of resistance to FHB in Sichuan wheat varieties,and breed wheat for resistance to FHB.【Method】In the research,156 wheat varieties or high⁃generation lines with moderate resistance to FHB were identified by soil surface inoculation.The resistance gene composition of these wheat varieties was detected by molecular markers of FHB resistance genesFhb1,Fhb2,Fhb4andFhb5,as well as some stripe rust resistance genes and powdery mildew resistance gene.【Result】Through molecular detection of disease resistance genes,it was found that 20yin979 and 20jian2506⁃10 contained the FHB resistance genes Fhb1andFhb5,respectively.For stripe rust resistance genes detection,4,63,9,3 and 14 wheat materials containing theYr5,Yr15,Yr18,Yr26andYrAs2388were detected,respectively.And the distribution frequencies of rust resistance genesYr5,Yr15,Yr18,Yr26and YrAs2388were 2.56%,40.38%,5.77%,1.92%and 8.97%,respectively.A total of 44 materials containing the powdery mildew resist⁃ance genePm21were detected,and the distribution frequency in the tested materials was 28.21%.The distribution of resistance genes showed that 122 materials contained at least one resistance gene.Among them,6 materials contained both strip rust and powdery mildew re⁃sistance genes,9 materials contained 2 strip rust resistance genes,and the remaining 106 materials contained only 1 resistance gene.The on⁃ly material 20jian2506⁃10 contained three resistance genes of FHB,stripe rust and powdery mildew.【Conclusion】These results indicate that Sichuan wheat lacks FHB resistance resources with explicit genetic background.Therefore,Sichuan wheat FHB resistance breeding should strengthen the introduction of known resistance resources while making full use of the local FHB resistance resources.

关 键 词：小麦 小麦赤霉病 Fhb基因 分子检测 抗病育种 

分 类 号：S512.1[农业科学—作物学]