乙烯通过调控氧化还原水平和耐冷基因表达提高早稻秧苗耐低温胁迫能力  

Ethylene Improves Low Temperature Tolerance of Early Rice Seedlings by Regulating REDOX Level and Cold Tolerance Gene Expression

作　　者：徐青山 张均华 魏倩倩 孔亚丽 潘林 黄晶 曹小闯 朱练峰 田文昊 金千瑜 朱春权 XU Qingshan;ZHANG Junhua;WEI Qianqian;KONG Yali;PAN lin;HUANG Jing;CAO Xiaochuang;ZHU Lianfeng;TIAN Wenhao;JIN Qianyu;ZHU Chunquan(State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China;School of Resources and Environmental Engineering,Anhui University,Hefei 230039;College of Life Sciences and Technology,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157000,China)

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,杭州310006 [2]安徽大学资源与环境工程学院,合肥230039 [3]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157000

出　　处：《土壤》2023年第1期153-160,共8页Soils

基　　金：国家自然科学基金项目(31901452,31872857);浙江省重点研发计划项目(2021C02063-3)资助。

摘　　要：乙烯参与调控植物响应多种逆境胁迫,然而乙烯调控早稻秧苗响应低温胁迫的生理和分子机制还不明确。本研究选择无土基质、发酵基质和营养土3种育秧基质对中早39早稻品种进行正常育秧至两叶一心期,外源喷施乙烯利后将水稻秧苗进行3d的低温处理,随后测定秧苗理化性质和抗寒基因表达,同时以喷施等量清水作为对照。结果显示:喷施乙烯利后显著提高了低温条件下水稻秧苗的生长,提高了秧苗体内的养分含量和渗透调节物质脯氨酸和可溶性糖含量,增强了秧苗体内抗氧化系统酶活性和氧化还原状态(AsA/DHA比率),提高了秧苗体内OsCOIN、TERF2、OsLti6a和OsCDPK7等耐冷基因的表达。以上结果说明,外源喷施乙烯通过调控水稻体内渗透调节物质含量、氧化还原水平和耐冷基因的表达增强水稻秧苗的耐低温胁迫能力。Ethylene is involved in regulating plant response to multiple stress.However,the physiological and molecular mechanisms by which ethylene regulates the response of early rice seedlings to low temperature stress remain unclear.In this study,three substrates including soilless substrate,fermentation substrate and nutritional soil were selected for raising early rice Zhongzao39 to the two-leaf stage.After being sprayed ethephon,rice seedlings were treated with low temperature for 3 days,and then the physiochemical properties and the expression of cold resistance genes were determined.Equal amount of water was sprayed as control.The results show that ethephon treatment significantly increases the growth of rice seedlings at low temperature,increases the contents of nutrients and osmotic regulation substances proline and soluble sugar,and enhances the antioxidant system enzyme activities and REDOX status(AsA/DHA ratio)of rice seedlings.The expression of cold tolerance genes such as OsCOIN,TERF2,OsLti6a and OsCDPK7 in seedlings are improved.These results suggest that ethylene spraying can enhance the resistance of rice seedlings to low temperature stress by regulating the content of osmotic substances,REDOX level and expression of cold tolerance genes in rice.

关 键 词：乙烯 基质 水稻 低温 基因 抗氧化酶 

分 类 号：S184[农业科学—农业基础科学]