长链非编码RNAHAGLR与微RNA-19a-3p对股骨颈骨折内固定术后骨折不愈合的影响  

Effects of long non-coding RNA HAGLR and microRNA-19a-3p on bone nonunion after internal fixation of femoral neck fra ctures

作　　者：马力[1] 汪盛玉 韩军烨 付豪 MA Li;WANG Shengyu;HAN Junye;FU Hao(Songzi People’s Hospital,Songzi 434200,Hubei,China)

机构地区：[1]松滋市人民医院,湖北松滋434200

出　　处：《中医正骨》2023年第4期1-6,共6页The Journal of Traditional Chinese Orthopedics and Traumatology

摘　　要：目的:观察长链非编码RNA(longnon-coding RNA,lncRNA)HAGLR与微RNA(microRNA,miRNA)-19a-3p对股骨颈骨折内固定术后骨折不愈合的影响。方法:回顾性分析采用切开复位AO中空加压螺钉内固定治疗的股骨颈骨折患者的病例资料,包括性别、年龄、基础疾病(高血压、糖尿病、冠心病)、损伤类型、骨折类型、骨折愈合情况,以及采用实时定量PCR检测法测得的外周血液中lncRNA HAGLR和miRNA-19a-3p表达量。按照术后骨折愈合情况分为愈合组和不愈合组。先对2组患者的相关信息进行单因素分析,再采用Logistic回归分析研究骨折不愈合的影响因素,最后采用受试者操作特征(receiver operating characteristic,ROC)曲线分析评价外周血液中lncRNA HAGLR与miRNA-19a-3p表达量对骨折不愈合的预测价值。结果:①骨折不愈合影响因素的单因素分析结果。共纳入141例患者,男73例、女68例。愈合组120例,不愈合组21例。2组患者年龄、损伤类型、骨折类型的比较,组间差异均有统计学意义(t=3.395,P=0.001;χ^(2)=16.904,P=0.000;χ^(2)=4.727,P=0.030)。术后4周、8周、12周,愈合组外周血液中lncRNAHAGLR表达量均高于不愈合组(0.79±0.20,0.63±0.15,t=3.494,P=0.001;0.87±0.17,0.75±0.16,t=3.009,P=0.003;0.95±0.16,0.87±0.19,t=2.054,P=0.042),miRNA-19a-3p表达量均低于不愈合组(1.19±0.29,1.40±0.30,t=3.046,P=0.001;1.13±0.25,1.27±0.27,t=2.340,P=0.003;1.03±0.22,1.18±0.20,t=2.919,P=0.042)。②骨折不愈合影响因素的多因素分析结果。将损伤类型、骨折类型以及术后4周外周血液中lncRNA HAGLR和miRNA-19a-3p表达量作为自变量,将骨折是否愈合作为因变量,进行Logistic回归分析,结果显示高能量损伤、骨折移位、术后4周外周血液中miRNA-19a-3p表达量是股骨颈骨折内固定术后骨折不愈合的危险因素(β=2.582,P=0.005,OR=1.423;β=1.734,P=0.011,OR=1.272;β=136.44,P=0.001,OR=23.698),术后4周外周血液中lncRNAHAGLR表达量是股骨颈骨折�Objective:To observe the ffects of long non-coding RNA(lncRNA)HAGLR and microRNA(miRNA)-19a-3p on bone nonunion after internal fixation of femoral neck fractures.Methods:The medical data of patients with femoral neck fractures treated with open reduction and internal fixation using AO hollow compression screws were retropectively analyzed,including gender,age,underlying diseases(hypertension,diabets,and coronary heart disease),injury type,fracture type,and fracture healing outcome.Real-tine quantitative PCR was used to detect the expression levels of lneRNA HAGLR and miRNA-19a-3p in the peripheral blood.The patients were divided into a union group and a nonunion group according to the postoper ative fracture healing outcomes.Firstly,univariate analysis was performed on the relevant information of patients in the two groups,fllowed by Logistic regression analysis for the influencing factors of bone nonunion.Fnally,the receiver operating characteristic(ROC)eurve was used to analye and evaluate the predictive value of the expression levels of IncRNA HAGIR and mi RNA-19a-3p in the peripheral blood for bone nonunion.Resuts:①Univariate analysis results of factors afetinrg bone nonunion.A total of 141 patients were involved,including 73 males and 68 females.There were 120 patients in the union group and 21 patients in the nonunion group.There were statistically significant differences in age,injury type,and fracture type betwcen the two groups(t=3.395,P=0.001;=16.904,P=0.000;χ^(2)=4.727,P=0.030).At4,8,and 12 weeks after surgery,the expression levels of IncRNA HAGLR in the periperal blood of the union group were higher than those in the nonunion group(0.79±0.20 vs0.63±0.15,t=3.494,P=0.001;0.87±0.17 vs0.75±0.16,t=3.009,P=0.003;0.95±0.16 vs 0.87±0.19,t=2.054,P=0.042),while the e pression levels of miRNA-19a-3p in the union group were lower than those in the nonunion group(1.19±0.29 vs 1.40±0.30,t=3.046,P=0.001;1.13±0.25 vs1.27±0.27,t=2.340,P=0.003;1.03±0.22 vs 1.18±0.20,t=2.919,P=0.042).②Multivariate anal-

关 键 词：股骨颈骨折 骨折固定术 内 骨折 不愈合 RNA 长链非编码 微RNAS 

分 类 号：R687.3[医药卫生—骨科学]