SCF/c-Kit信号通路通过抑制肥大细胞增殖调控影响病理性瘢痕形成的研究  

SCF/c-Kit Signaling Pathway Affects Pathological Scar Formation by Inhibiting Mast Cell Proliferation Regulation

作　　者：吴海燕[1] 王永军 段志娟[1] 王娟 罗宏 Wu Haiyan;Wang Yongjun;Duan Zhijuan;Wang Juan;Luo Hong(Chenzhou First People's Hospital,Chenzhou 423000,China)

机构地区：[1]郴州市第一人民医院,湖南郴州423000

出　　处：《中国中西医结合皮肤性病学杂志》2023年第2期107-112,共6页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

摘　　要：目的探讨配体干细胞因子/c-KIT原癌基因(SCF/c-kit)对肥大细胞增殖的影响,并进一步探讨其在病理性瘢痕形成中的作用。方法收集病理性瘢痕组织和正常组织,通过甲苯胺蓝染色法检测组织中肥大细胞数目,酶联免疫吸附测定(ELISA)法检测患者血清中干细胞因子SCF的表达,蛋白质印迹法(Western blotting)检测组织中SCF和其受体c-Kit的表达。建立病理性瘢痕裸鼠模型,16 d后将造模成功的裸鼠随机分为模型+沉默阴性对照(si-NC)组和模型+si-SCF组,分别于裸鼠瘢痕内注射si-NC、si-SCF质粒和脂质体混合液0.25 mL,另设对照组,注射等体积磷酸盐缓冲液(PBS)。7 d后收集标本,游标卡尺测量裸鼠瘢痕体积大小,V.G染色检测瘢痕组织Ⅰ型胶原含量,甲苯胺蓝染色评估瘢痕组织肥大细胞数目,Western blotting检测组织中SCF和c-Kit的表达。结果病理性瘢痕患者的瘢痕组织出现大量肥大细胞,同时在病理性瘢痕患者血清中发现SCF高表达,而且在其瘢痕组织中SCF和c-Kit蛋白表达也增多;在病理性瘢痕模型裸鼠体内沉默SCF,抑制了c-Kit的表达,减小了模型裸鼠的瘢痕体积,降低了瘢痕组织中Ⅰ型胶原含量,还抑制了肥大细胞增殖。结论SCF/c-Kit信号通路可能是通过抑制肥大细胞增殖,来调控病理性瘢痕的形成。SCF/c-Kit的表达失控可能也是病理性瘢痕形成的一个重要诱因。Objective In order to investigate the effects of stem cell factor/c-KIT proto-oncogene(SCF/c-kit)on the proliferation of mast cells,and further explore its role in pathological scar formation.Methods Pathological scar tissue and normal tissue were collected,and the number of mast cells in the tissue was detected by toluidine blue staining.The expression of stem cell factor SCF in the serum of patients was detected by enzyme linked immunosorbent assay(ELISA),and the expression of SCF and its receptor c-Kit in the tissue were detected by Western blotting.A model of pathological scarred nude mice was established.After 16 days,the nude mice successfully modeled were randomly divided into model+siRNA negative control(si-NC)group and model+si-SCF group,and 0.25 mL of the mixed solution with liposomes with si-NC or si-SCF plasmids were injected into the scars of nude mice.For control group,an equal volume of phosphate buffered saline(PBS)was injected.After seven days,the specimens were collected,vernier calipers were used to measure the scar volume of nude mice,V.G staining was used to detect the typeⅠcollagen content in scar tissue,toluidine blue staining was used to assess the number of mast cells in the scar tissue,and Western blotting was used to detect the expression of SCF and c-Kit in the tissue.Results A large number of mast cells appeared in the scar tissue of patients with pathological scars.At the same time,high expression of SCF was found in the serum of patients with pathological scars,and the expression of SCF and c-Kit protein in their scar tissues also increased;in the pathological scar model nude mice knocking down SCF inhibited the expression of c-Kit,reduced the scar volume of model nude mice,reduced the content of typeⅠcollagen in scar tissue,and inhibited the proliferation of mast cells.Conclusion The SCF/c-Kit signaling pathway may regulate the formation of pathological scars by inhibiting mast cell proliferation.The uncontrolled expression of SCF/c-Kit may also be an important cause of patholo

关 键 词：配体干细胞因子/酪氨酸激酶受体c 肥大细胞 细胞增殖 病理性瘢痕 

分 类 号：R619.6[医药卫生—外科学]