玉米紫色叶突变体zmpl-1表型分析及基因定位  

Phenotype Analysis and Gene Mapping of Maize Purple Leaf Mutant zmpl-1

作　　者：朱振兴[1] 郭长英[1] 李丹[1] ZHU Zhen-xing;GUO Chang-ying;LI Dan(Crop Molecular Improvement Lab,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161)

机构地区：[1]辽宁省农业科学院作物分子改良实验室,辽宁沈阳110161

出　　处：《辽宁农业科学》2023年第2期7-12,共6页Liaoning Agricultural Sciences

基　　金：辽宁省优秀青年自然基金项目(2021-YQ-04);中国博士后面上基金项目(2021M693848)

摘　　要：花青素是一类广泛存在于植物中的水溶性天然色素,因其良好的抗氧化功能,也是重要保健化合物,因此发掘玉米花青素分子代谢途径有重要价值。试验对玉米紫色叶突变体zmpl-1进行了表型分析、叶片花青素含量测定、遗传和重测序BSA-seq基因定位分析。结果表明,紫色叶性状在老叶中更严重;突变体紫叶中花青素含量较野生型大幅提高;紫色叶性状可能受3个隐性基因共同调控;重测序BSA-seq定位将该紫色叶性状控制基因定位在4条染色体上207.3 Mb的区间内,包括染色体第2条、第10条、第3条以及第5条。该研究为解析玉米花青素形成分子机制提供了参考。Anthocyanin is a class of soluble natural pigments that widely exists in plants.Anthocyanin also is an important health protection compound because of its good antioxidant property.Thus,it is of great value to explore molecular metabolic pathways of maize anthocyanin.Maize purple leaf mutant was studied through phenotype analysis,anthocyanin concentration determination,genetic analysis,and resequencing based BSAseq mapping.Results showed that symptom of purple leaf was more severe in older leaves.The mutant purple leaf anthocyanin concentration was significantly higher than that of the wild type.The purple leaf might be controlled by three recessive genes.BSA-seq mapping showed that the purple leaf controlling genes were located in an interval of 207.3 Mb on four chromosomes,including chromosome 2,10,3 and 5.The study of this specific maize purple leaf mutant provides important data for analysis the new molecular mechanism of maize anthocyanin biosynthetic pathway.

关 键 词：花青素 紫色叶 叶色 突变体 玉米 

分 类 号：S513.035.2[农业科学—作物学]