Assembly and analysis of the Populus deltoides mitochondrial genome:the first report of a multicircular mitochondrial conformation for the genus Populus  

作　　者：Yanshu Qu Pengyan Zhou Chunfa Tong Changwei Bi Li’an Xu 

机构地区：[1]Key Laboratory of Forestry Genetics&Biotechnology of Ministry of Education,Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,People’s Republic of China [2]College of Information Science and Technology,Nanjing Forestry University,Nanjing 210037,People’s Republic of China

出　　处：《Journal of Forestry Research》2023年第3期717-733,共17页林业研究（英文版）

基　　金：funded by the National Key Research and Development Program of China[Grant Number 2021YFD2201205];the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

摘　　要：Genomics research of Populus deltoides,an important timber species that is widely planted worldwide,is an important part of poplar breeding.Currently,the nuclear and chloroplast genome of P.deltoides have been sequenced,but its mitochondrial genome(mitogenome)has not been reported.To further explore the evolution and phylogeny of P.deltoides,the mitogenome of P.deltoides I-69 was assembled using reads from Nanopore and Illumina sequencing platforms and found to consist of 802,637 bp and three circular chromosomes(336,205,280,841,and 185,591 bp)containing 58 genes(34 protein-coding genes,21 tRNA genes,and 3 rRNA genes).RNA analysis in combination with several species showed signifi cantly fewer RNA editingsites in the mitogenomes of poplar and other angiosperms than in gymnosperms.Sequence transfer analysis showed extensive mitogenome rearrangements in Populus species,and with evolution from lower to higher plants,tRNA transfer from chloroplasts to mitochondria became increasingly frequent.In a phylogenetic analysis,the evolutionary status of P.deltoides was determined,and the section Populus was supported.Our results based on the fi rst report of a multicircular conformation of the Populus mitogenome provide a basis for further study of the evolution and genetics of P.deltoides and other Populus species and for breeding programs.

关 键 词：Mitochondrial genome Multi-circular Populus deltoids Comparative genomics PHYLOGENY 

分 类 号：S792.11[农业科学—林木遗传育种]