肠道微生物组与非吸烟女性肺癌关联研究  

Association of gut microbiome with lung cancer in non-smoking women

作　　者：张帅 郑萍[1] 许宁[1] 王强[1] ZHANG Shuai;ZHENG Ping;XU Ning;WANG Qiang(China CDC Key Laboratory of Environment and Population Health/National Institute of Environmental Health,Chinese Center for Disease Control and Prevention,Beijing 100021,China)

机构地区：[1]中国疾病预防控制中心环境与人群健康重点实验室中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《环境卫生学杂志》2023年第4期234-242,共9页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：国家卫生健康委中央财政项目(131031108000160005)。

摘　　要：目的探索肠道菌群变化与非吸烟女性肺癌风险的关联,为非吸烟女性肺癌的防控提供干预依据。方法采用以医院为基础的多中心病例对照研究,使用16S rRNA基因测序技术对所收集的192例研究对象(病例组n=92;对照组n=100)的粪便样本进行测序,利用多样性云分析平台分析肺癌患者肠道菌群特征。运用LASSO回归和Logistic回归分析两组差异肠道菌群分值与非吸烟女性肺癌的关联。结果病例组和对照组肠道菌群丰富度(Wilcoxon检验,P<0.05)和β多样性的差异(ANOSIM分析,P=0.01)均有统计学意义。经LEfSe分析,病例组和对照组共有22个差异菌属,其中病例组特征菌属为拟杆菌属(Bacteroides)、副拟杆菌属(Parabacteroides)、乳梭菌属(Lachnoclostridium)、无害芽孢梭菌属(Clostridium innocuum group)、罗姆布茨菌属(Romboutsia)等11个菌属,对照组特征菌属为Clostridium sensu stricto 1、Agathobacter、多利菌属(Dorea)、柯林斯菌属(Collinsella)、UCG-002等11个菌属。经过LASSO回归和Logistic回归分析,差异菌群的菌群分值与肺癌风险为正关联,是肺癌的危险因素(AOR=2.22,95%CI:1.63~3.02)。此外,居住环境(曾长期居住在地下室或一楼),腌菜、肉类和酸奶食用频率也与非吸烟女性肺癌的发生相关,其中曾长期居住在地下室或一楼、食用肉类和腌菜频率高是肺癌发生的危险因素,食用酸奶频率高是保护因素。结论肺癌患者与非肺癌对照人群肠道菌群结构存在一定的差异,肠道微生态变化可能是增加非吸烟女性肺癌患病风险的危险因素。Objective To investigate the association of alterations in gut microbiota with the risk of lung cancer in non-smoking women,and provide an evidence for the prevention and control of lung cancer in non-smoking women.Methods A hospital-based multicenter case-control study was conducted.The 16S rRNA sequencing technique was used for sequencing of stool samples collected from 192 subjects,who were divided into lung-cancer case group with 92 subjects and control group with 100 subjects,and the diversity cloud analysis platform was used to analyze the features of gut microbiota in lung cancer patients.The LASSO regression analysis and the logistic regression analysis were used to investigate the association of the weighted scores of distinct gut microbiota with the risk of lung cancer in non-smoking women.Results There were significant differences between the case group and the control group in the abundance(Wilcoxon test,P<0.05)andβdiversity(ANOSIM,P=0.01)of gut microbiota.The LEfSe analysis identified 22 differentially expressed bacterial genera between the case group and the control group,among which 11 genera including Bacteroides,Parabacteroides,Lachnoclostridium,Clostridium innocuum group,and Romboutsia were the characteristic bacterial genera in the case group,while 11 genera including Clostridium sensu stricto 1,Agathobacter,Dorea,Collinsella,and UCG-002 were the characteristic bacterial genera in the control group.The LASSO and logistic regression analyses showed that the scores of the differentially expressed flora were positively associated with the risk of lung cancer(AOR=2.22,95%confidence interval:1.63-3.02).In addition,living environment(living in basement or on the first floor for a long time)and frequency of intake of pickles,meat,and yogurt were also associated with the onset of lung cancer in non-smoking women,among which living in basement or on the first floor for a long time and high frequency of intake of pickles and meat were the risk factors for lung cancer,while high frequency of intake of yo

关 键 词：非吸烟女性肺癌 肠道微生物组 菌群分值 16S rRNA QIIME2 LASSO 

分 类 号：R12[医药卫生—环境卫生学]