辣椒疫霉菌拮抗细菌的分离鉴定及防效试验  

Isolation and identification of antagonistic bacteria against Phytophthora capsici and its antagonistic efficacy

作　　者：高香辉 朱丽娜 谭泽宝 郑井元[2] 严蓓 刘敏 罗坤[1] GAO Xianghui;ZHU Lina;TAN Zebao;ZHENG Jingyuan;YAN Bei;LIU Min;LUO Kun(College of Plant Protection,Hunan Agricultural University,Changsha,Hunan 410128,China;Institute of VegetableResearch,Hunan Academy of Agricultural Sciences,Changsha,Hunan 410125,China;Economic Specialties Station,Wucheng District,Jinhua,Zhejiang 321000,China)

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]湖南省农业科学院蔬菜研究所,湖南长沙410125 [3]金华市婺城区经济特产站,浙江金华321000

出　　处：《湖南农业大学学报（自然科学版）》2023年第2期201-205,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省教育厅项目(20C0958、20A262);浙江省金华市科学技术局项目(2020-2-007)。

摘　　要：在辣椒疫病为害较严重田块采集健康植株,从其根际土壤中分离筛选出具有抗疫霉菌活性的细菌菌株Pa608。通过菌落形态特征以及分子水平同源性鉴定,测定其生理生化指标和胞外产酶特性,确定该菌株为铜绿假单胞菌(Pseudomonas aeruginosa);采用平板对峙法测试其抗菌谱,盆栽试验确定防效后应用于温室辣椒田测试温室防效,发现该菌株对灰霉菌、稻梨孢菌、疫霉菌、炭疽杆菌具有较好的拮抗作用,当菌液浓度为1.0×10^(9)cfu/mL、盆施3 mL对辣椒疫病的盆栽防效达88.00%;菌液稀释10倍、株施50 mL的温室防效达74.96%。The bacterial strain Pa608 was isolated and screened from the inter-rhizosphere soil of healthy plants collected from fields with severe pepper blight damage,and was identified as Pseudomonas aeruginosa through colony morphology,molecular homology and its physiological,biochemical and extracellular enzyme-producing characteristics.The antimicrobial spectrum of Pseudomonas aeruginosa was tested by the plate standoff method,and the efficacy of the antimicrobial spectrum was determined in pot tests,and then applied to greenhouse pepper fields to test its greenhouse efficacy.It is found that the strain has good antagonistic effect on Botrytis cinerea,Pyricularia oryzae,Phytophthora capsici,Bacillus anthracis,etc.The efficacy was 88.00%in potted plants at a concentration of 1.0×10^(9)cfu/mL,pouring 3 mL/pot,and the efficacy was 74.96%in conservatories at the tenfold dilution of the bacterial solution and pouring 50 mL/plant.

关 键 词：辣椒疫病 拮抗菌 分离 鉴定 防效 

分 类 号：S436.418.1[农业科学—农业昆虫与害虫防治]