睡菜水提液对小鼠的急性毒性实验研究  

Research of Acute Toxicity for Water Extract from Menyanthes Trifoliata L in Mice

作　　者：魏雪娇 石松云 毛楚涵 何婷 刘呈雄 陈剑锋 贺海波 黄文峰[3] 邹坤 程凡 WEI Xuejiao;SHI Songyun;MAO Chuhan;HE Ting;LIU Chengxiong;CHEN Jianfeng;HE Haibo;HUANG Wenfeng;ZOU Kun;CHENG Fan(Key Laboratory of Natural Product Research and Development,College of Biological&Pharmaceutical Sciences,China Three Gorges Univ.,Yichang 443002,China;Yichang Key Laboratory of Development and Utilization of Health Products with Drug Food Homology,College of Biological&Pharmaceutical Sci-ences,China Three Gorges Univ.,Yichang 443002,China;College of Medicine,China Three Gorges Univ.,Yichang 443002,China)

机构地区：[1]三峡大学生物与制药学院,天然产物研究与利用湖北省重点实验室(三峡大学),湖北宜昌443002 [2]三峡大学生物与制药学院,药食同源大健康产品开发利用宜昌市重点实验室,湖北宜昌443002 [3]三峡大学医学院,湖北宜昌443002

出　　处：《三峡大学学报（自然科学版）》2023年第3期108-112,共5页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金(81803383,81773952)。

摘　　要：目的:观察睡菜水提液对大鼠的急性毒性.方法:预实验:灌胃给予小鼠水提液剂量分别为2455、1841.25、1380.94、1035.70 g/L,观察给药当天及7 d内小鼠的状态,计算半数致死量(50%lethal dose,LD_(50));最大耐受量实验:灌胃给予睡菜最大剂量2455 g/L,观察给药后14 d内小鼠状态和死亡情况,给药14 d后检测小鼠的血液生化指标、计算主要脏器指数以及观察主要脏器病理学变化.结果:未能测出睡菜水提液的半数致死量.最大耐受量实验显示:给药后14 d内小鼠状态良好,无中毒反应或死亡情况.给药组小鼠第3、7、11、14 d体质量增长与空白组比较无统计学差异(P>0.05);给药组小鼠血液生化指标、主要脏器指数与空白组无统计学差异(P>0.05);组织形态学、组织病理学检查未发现睡菜水提液对小鼠主要脏器产生病理改变.结论:睡菜水提液的最大无毒剂量大于2455 g/L,是成人临床用药(15 g)的100.6倍,是安全的用药剂量范围.Objective:To observe the acute toxicity of Menyanthes trifoliata L in mice.Methods:Pre-test:mice were given the aqueous extract at doses of 2455,1841.25,1380.94 and 1035.70 g/L by gavage,and the status of mice was observed on the day of administration and within 7 d.The LD_(50)(50% lethal dose) was calculated;the maximum tolerance test:The mice were given 2455 g/L by gavage,and the status and death of mice were observed after the administration of the drug within 14 d.Results:The LD_(50) values of the aqueous extract of sleepy vegetable could not be measured.The maximum tolerance test indicated that the mice were in good status within 14 d after administration,and there was no toxic reaction or death.There was no statistical difference in the increase of body mass of mice in the 3rd,7th,11th and 14th day of administration compared with the control group(P>0.05);there were no statistical difference in the blood biochemical index and main organ index of mice in the administration group compared with the control group(P>0.05);the histomorphology and histopathology examination did not reveal any pathological changes in the main organs of mice caused by the aqueous extract of water hyacinth.Conclusion:The maximum non-toxic dose of aqueous extract of water hyacinth is greater than 2455 g/L,which was 100.6(15 g) times of the clinical dose for adults and was a safe dose range.

关 键 词：睡菜 小鼠 急性毒性实验 

分 类 号：R93[医药卫生—生药学]