基于非靶向代谢组学的片烟醇化进程判断及代谢物差异分析  

Evaluation and differential metabolite analysis of tobacco maturation based on non-targeted metabolomics

作　　者：梁珂馨 奚家勤[1] 陈若星 牟文君[1] 刘萍萍[1] 郑庆霞[1] 王得强 贾中林 李晓闯 张艳玲[1] LIANG Kexin;XI Jiaqin;CHEN Ruoxing;MU Wenjun;LIU Pingping;ZHENG Qingxia;WANG Deqiang;JIA Zhonglin;LI Xiaochuang;ZHANG Yanling(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;Technology Center of China Tobacco Jiangxi Industrial Co.,Ltd.,Nanchang 330096,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]江西中烟工业有限责任公司技术中心,南昌330096

出　　处：《烟草科技》2023年第4期17-25,共9页Tobacco Science & Technology

基　　金：中国烟草总公司重点研发项目“品牌导向型高可用性上部烟叶开发与高效利用技术研究”(110202102035);江西中烟工业有限责任公司科技项目“江西赣州仓库烟叶醇化及调控技术研究”(2020360000340109);重庆中烟工业有限责任公司科技项目“重庆中烟中支卷烟核心原料醇化调控技术研究”(2022500000300091);广东中烟工业有限责任公司科技项目“广东韶关仓库片烟醇化调控技术研究”(2021440000340015)。

摘　　要：为探究非靶向代谢物在烟叶醇化过程中的变化规律,利用基于GC-MS法的非靶向代谢组学技术对云南省3个产区(曲靖、红河和昆明)的上部和中部烟叶进行分析。结果表明:醇化过程中,烟叶中部分糖类、氨基酸类和多酚类物质相对含量总体上呈下降趋势,有机酸类物质相对含量总体上呈升高趋势。基于非靶向代谢物组成,烟叶醇化进程可分为3个阶段,云南上部和中部烟叶适宜醇化期分别为21~33个月和21~27个月。利用偏最小二乘判别分析(PLS-DA),筛选出醇化前期至醇化中期的差异代谢物共16种,主要富集在糖代谢通路中;醇化中期至醇化后期的差异代谢物共10种,主要富集在有机酸代谢通路中。In order to investigate the change of non-targeted metabolites during tobacco maturation process,the upper and middle tobacco leaves from three producing areas(Qujing,Honghe,and Kunming)in Yunnan Province were studied by combining non-targeted metabolomic analysis with gas chromatography-mass spectrometry(GC-MS).The results showed that the relative contents of some sugars,amino acids,and polyphenols in tobacco leaves decreased generally during the aging process,while those of organic acids increased generally.Based on the constitution of the non-targeted metabolites,tobacco maturation could be divided into three stages.The suitable aging periods for the upper and middle leaves of Yunnan tobacco were 21-33 months and 21-27 months respectively.Using partial least squares discriminant analysis(PLS-DA),16 differential metabolites were screened from early to middle aging stages,which were mainly enriched in the metabolic pathways of carbohydrates.Further 10 differential metabolites were screened from middle to late aging stages,which were mainly enriched in the metabolic pathways of organic acids.

关 键 词：片烟醇化 代谢组学 差异代谢物 

分 类 号：TS411.1[农业科学—烟草工业]