基于PI3K/Akt/mTOR信号通路的苦参碱诱导人乳腺癌MCF-7细胞自噬及其机制的研究  

Research on Matrine Induced Autophagy of Human Breast Cancer MCF-7 Cells Based on PI3K/Akt/mTOR Signal Pathway and Its Mechanism

作　　者：贾绍华[1] 丁海鑫 孙萌遥 金诗鹏 JIA Shao-hua;DING Hai-xin;SUN Meng-yao;JIN Shi-peng(School of Pharmacy(Pharmaceutical Engineering Technology Research Center),Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学药学院(药物工程技术研究中心),黑龙江哈尔滨150076

出　　处：《食品与药品》2023年第2期159-163,共5页Food and Drug

基　　金：黑龙江省自然科学基金重点项目(编号:ZD2018016)。

摘　　要：目的 研究苦参碱对人乳腺癌MCF-7细胞自噬及其相关信号通路的影响,为苦参碱的抗肿瘤作用提供更多依据。方法 采用自噬双标腺病毒(mRFP-GFP-LC3)法、透射电镜法(TEM)检测给药后细胞自噬水平的变化;Western blot检测苦参碱对自噬相关蛋白LC3、Beclin-1,信号通路蛋白PI3K、p-PI3K、Akt、p-Akt、mTOR、p-mTOR表达水平的影响。结果 随着苦参碱浓度的上升,细胞中的自噬水平也在逐渐升高,自噬相关蛋白LC3-II/LC3-I、Beclin-1的表达量呈上升趋势,通路相关蛋白PI3K、Akt、mTOR无明显变化,p-PI3K、p-Akt、p-mTOR的表达量均呈现下降趋势。结论 苦参碱可剂量依赖性升高MCF-7细胞的自噬水平,机制可能与PI3K/Akt/mTOR介导的信号通路有关。Objective To study the effects of matrine on autophagy and related signaling pathways of human breast cancer MCF-7 cells,and to provide more evidence for the antitumor effect of matrine.Methods Autophagy double labeled adenovirus(mRFP-GFP-LC3) and transmission electron microscopy(TEM) were used to detect the changes of autophagy level.Western blot was used to detect the effect of matrine on the expression of autophagy related protein LC3,Beclin-1,and signal pathway proteins including PI3K,p-PI3K,Akt,p-Akt,mTOR and p-mTOR.Results With the increase of matrine level,the autophagy level in cells increased gradually.The expression of autophagy related proteins LC3-II/LC3-I and Beclin-1 showed an upward trend.The expression of pathway related proteins PI3K,Akt and mTOR did not change significantly.The expression of p-PI3K,p-AKT and p-mTOR showed a downward trend.Conclusion Matrine can increase the autophagy level of MCF-7 cells in a dose-dependent manner.The mechanism may be related to the PI3K/Akt/mTOR mediated signal pathway.

关 键 词：苦参碱 人乳腺癌MCF-7细胞 细胞自噬 作用机制 

分 类 号：R285[医药卫生—中药学]