洋甘菊中2种咖啡酰基奎宁酸大孔树脂纯化工艺的优化  

Optimization of Purification Process of Two Cafeoyl Quinic Acids by Macroporous Resins from Chamomile

作　　者：张柏生[1,2] 热依木古丽·阿布都拉 信学雷[3] 范芳芳[1,2] 李茜[1,2] ZHANG Bo-sheng;Rahima Abdulla;XIN Xue-lei;FAN Fang-fang;LI Qian(Hospital of Chinese Medicine Affiliated to Xinjiang Medical University,Urumqi 830000,China;Xinjiang Key Laboratory of Processing Research of Traditional Chinese Medicine,Urumqi 830000,China;CAS Key Laboratory of Chemistry of Plant Resources in Arid Regions,State Key Laboratory Cultivation Base Jointly Built by Province and Ministry for Utilization of Xinjiang Indigenous Medicinal Plants Resource,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China)

机构地区：[1]新疆医科大学附属中医医院,新疆乌鲁木齐830000 [2]新疆中药炮制研究重点实验室,新疆乌鲁木齐830000 [3]中国科学院干旱区植物资源化学重点实验室,新疆特有药用资源利用省部共建国家重点实验室培育基地,中国科学院新疆理化技术研究所,新疆乌鲁木齐830011

出　　处：《食品与药品》2023年第2期I0014-I0019,共6页Food and Drug

基　　金：新疆维吾尔自治区自然科学基金(编号:2021D01E32);乌鲁木齐市科技局优秀青年科技人才项目。

摘　　要：目的优化洋甘菊中2种咖啡酰基奎宁酸大孔吸附树脂纯化工艺。方法以2种咖啡酰奎宁酸的吸附率和解吸率为评价指标筛选树脂种类。以吸附率和解吸率为评价指标,筛选最优工艺参数,包括上样液浓度、上样量、吸附速率、水冲洗用量、洗脱剂种类和用量等。结果最佳条件为采用AB-8大孔吸附树脂纯化,药液浓度0.20 g/ml,树脂柱径高比1:6,吸附速率2 BV/h,在此条件下,上柱量达7 BV;吸附好的树脂先用1 BV水洗净,流速5 BV/h,再用3 BV 50%乙醇和1 BV 70%乙醇洗脱活性部位,合并洗脱液,浓缩,干燥既得。结论该工艺稳定可靠,可用于大孔吸附树脂纯化洋甘菊中2种咖啡酰基奎宁酸。Objective To optimize the purification process of two kinds of cafeoyl quinic acids from chamomile by macroporous adsorption resin.Methods The adsorption and desorption rates of two kinds of caffeoyl quinic acids were used to select the resin types.The adsorption and desorption rates were used as evaluation indexes to screen the optimal process parameters,including the concentration and amount of the sample,the adsorption velocity,the amount of washing water,the type and amount of eluant,and so on.Results The optimal purification conditions were as follows:AB-8 macroporous adsorption resin was adopted for purification,the concentration of the sample was 0.20 g/ml,the ratio of diameter to height of the resin column was 1:6,the adsorption velocity was 2 BV/h.Under these conditions,the sample volume reached 7 BV.The adsorbed resin was washed with 1 BV of water at the flow rate of 5 BV/h,then eluted with 3 BV of 50%ethanol and 1 BV of 70%ethanol,then the eluates were combined,concentrated and dried.Conclusion The process is stable and reliable,and can be used for the purification of two caffeoyl quinic acids from chamomile by macroporous adsorption resin.

关 键 词：洋甘菊 3 5-二-O-咖啡酰基奎宁酸 4 5-二-O-咖啡酰基奎宁酸 大孔吸附树脂 纯化 

分 类 号：R284[医药卫生—中药学]