多浪羊PRLR基因克隆测序及不同组织差异表达分析  

Cloning Sequencing of PRLR Gene and Differential Expression Analysis of Different Tissues

作　　者：李孝君 隋志远 王晨光 张永杰 张志帅 邢凤[1,2] LI Xiaojun;SUI Zhiyuan;WANG Chenguang;ZHANG Yongjie;ZHANG Zhishuai;XING Feng(College of Animal Science,Tarim University,Aral 843300,Xinjiang,China;Tarim Animal Husbandry,Xinjiang Production and Construction Corps Point Laboratory,Aral 843300,Xinjiang,China)

机构地区：[1]塔里木大学动物科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科重点实验室,新疆阿拉尔843300

出　　处：《四川农业大学学报》2023年第2期344-351,共8页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金项目(31660652;31960655)。

摘　　要：【目的】克隆多浪羊催乳素受体基因(PRLR)CDS区序列,并检测不同组织中PRLR基因在初情期前后表达差异,为PRLR基因在初情期启动过程中发挥的主要作用提供参考依据。【方法】利用RT-PCR技术和使用相关的生物学信息软件预测其编码蛋白的结构和功能,通过qPCR检测多浪羊初情期前后各组织中的PRLR基因的表达水平。【结果】获得了多浪羊PRLR基因的CDs区序列长1 746 bp。多浪羊的3个时期5个组织中均有PRLR基因的表达。PRLR基因在初情期前多浪羊垂体组织中的表达量显著高于其他组织(P<0.05);初情期垂体组织中PRLR基因的表达量仍显著高于其他4个组织(P<0.05),初情期后下丘脑和垂体组织中PRLR基因的表达量显著高于其他3个组织(P<0.05);初情期垂体和输卵管组织中PRLR基因的表达量升高,初情期后显著高于初情期前和初情期后(P<0.05)。【结论】揭示了PRLR基因可能在多浪羊初情期启动的过程中参与了调控。【Objective】This study was conducted to clone the CDS sequence of prolactin receptor gene(PRLR)of Duolang sheep and detect the expression difference of PRLR gene before and after the initial estrus in different tissues,so as to provide reference for the main role of PRLR gene in the initiation of the initial estrus.【Method】RT-PCR technology and relevant biological information software were used to predict the structure and function of the encoded protein,and qPCR was used to detect the expression level of PRLR gene in each tissue of Duolang sheep before and after the first estrus stage.【Result】The CDs sequence of PRLR gene of Duolang sheep was 1746 bp in length.PRLR gene was expressed in all five tissues of Duolang sheep at three stages.The expression level of PRLR gene in pituitary tissues of Duolang sheep before estrus was significantly higher than that in other tissues(P<0.05).The expression level of PRLR gene in pituitary tissues was still significantly higher than that in the other four tissues(P<0.05),and the expression level of PRLR gene in hypothalamus and pituitary tissues was significantly higher than that in the other three tissues after the initial estrus(P<0.05).The expression of PRLR gene was increased in pituitary and oviduct tissues during the initial estrus period,and was significantly higher after the initial estrus period than before and after the initial estrus period(P<0.05).【Conclusion】These results suggest that PRLR gene may be involved in the regulation of estrus initiation in Duolang sheep.

关 键 词：多浪羊 PRLR 初情期 克隆 生物信息学分析 

分 类 号：S826.2[农业科学—畜牧学]