免疫去势与手术去势对SD雄鼠骨骼肌沉积的差异影响机制  

New Insights into the Differential Effects of Immunocastration Versus Surgical Castration on Skeletal Muscle Accumulation in Male Sprague-Dawley Rats

作　　者：李镇宏 李昊卿 李嘉欣 曾宪垠[1] 韩兴发[1] LI Zhenhong;LI Haoqing;LI Jiaxin;ZENG Xianyin;HAN Xingfa(College of Life Science,Sichuan Agricultural University,Ya’an 625014,Sichuan,China)

机构地区：[1]四川农业大学生命科学学院,四川雅安625014

出　　处：《四川农业大学学报》2023年第2期365-371,共7页Journal of Sichuan Agricultural University

基　　金：四川省苗子工程重点项目(2020JDRC0101)。

摘　　要：【目的】试验旨在以SD雄鼠为模型探讨免疫去势与手术去势对公畜骨骼肌沉积的差异影响机制。【方法】对比分析免疫去势与手术去势对雄鼠腓肠肌重量、组织学及其沉积相关激素及基因表达的影响。【结果】相较于免疫去势,雄鼠手术去势后血清IGF1降低,皮质酮升高,腓肠肌肌肉生长抑制素基因(Mstn)表达上调,血清尿素氮水平升高。【结论】相对较低的IGF1和较高的皮质酮是手术去势较免疫去势导致公畜骨骼肌分解萎缩更显著的重要原因。【Objective】 This study was aimed to explore the differential effects of immunocastration versus surgical castration on skeletal muscle accumulation as well as its underlying mechanisms using the male SD rat as model.【Method】 The gastrocnemius muscle mass,histology as well as its accumulationassociated hormone and gene expression level were analyzed comparatively between immunocastrated and surgically castrated male rats.【Result】 Compared to immunocastration,surgical castration caused lower serum IGF1 and higher corticosterone levels,higher gastrocnemius myostatin(Mstn) mRNA expression and higher serum urea nitrogen levels in male rats.【Conclusion】 Lower IGF1 and higher glucocorticoid levels were the important reasons to cause more skeletal muscle myolysis in surgically castrated males than that in immunocastrated males.

关 键 词：免疫去势 手术去势 骨骼肌 差异沉积 大鼠 

分 类 号：S828.2[农业科学—畜牧学]