川黄连黄酮类成分抗氧化活性研究  

Study on The Antioxidant Activity Of Flavonoids From Coptis Chinensis Franch

作　　者：秦瑞博 王洁雪 陈方梅 QIN Ruibo;WANG Jiexue;CHEN Fangmei(College of Chemistry and Life Science,Sichuan Provincial Key Laboratory for Structural Optimization and Application of Functional Molecules,Chengdu Normal University,Chengdu,611130,China)

机构地区：[1]成都师范学院化学与生命科学学院功能分子结构优化与应用四川省高校重点实验室,四川成都611130

出　　处：《工业微生物》2023年第2期32-36,共5页Industrial Microbiology

基　　金：成都师范学院校级科研项目(CS19ZA04)。

摘　　要：文章探究川黄连中黄酮类成分的抗氧化性能,采用正交实验设计法优化川黄连中黄酮类成分的提取工艺,并考查总黄酮提取物对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐阳离子自由基(ABTS+·)的清除率;提取川黄连中总黄酮的最佳工艺条件为:75%乙醇、料液比1∶15(g/mL)、回流提取时间1.5 h。在该工艺下,川黄连中黄酮的总含量为0.6768%。质量浓度分别为0.0036~0.02875 mg/m L、0.02875~0.2300 mg/mL,川黄连总黄酮对DPPH自由基和ABTS自由基的清除能力明显强于VC。川黄连总黄酮对两者均有较好的清除能力,且在一定浓度内体外抗氧化活性明显强于VC。从而得出川黄连黄酮类成分具有一定抗氧化能力的结论,说明生物碱之外的其他化学成分有较好的开发价值,本文为川黄连抗氧化活性的深入研究及开发利用提供了一定的参考。To investigate the antioxidant properties of flavonoids,the orthogonal experimental design was used to optimize the extraction process of flavonoids from,and the scavenging rates of total flavonoids on 1,1-Diphenyl-2-rinitrophenylhydrazine radical(DPPH·)and 2,2-Biazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diammonium salt cationic radical(ABTS+·)were investigated.The optimum extraction conditions were as follows:75% ethanol,solid-liquid ratio 1:15(g/mL),reflux extraction time 1.5 h.Under this,the total flavonoids content was 0.6768%.The scavenging ability of total flavonoids on DPPH and ABTS free radicals was significantly stronger than that of VC at 0.0036-0.02875 mg/mL and 0.02875-0.2300 mg/mL,respectively.The total flavonoids of Rhizoma Coptidis have good scavenging capacity for them,and the antioxidant activity in vitro is significantly stronger than that of VC within a certain concentration range.The flavonoids of Rhizoma Coptidis have certain antioxidant capacity,which indicates that other chemical components except alkaloids have good development value.The research in this article provides a theoretical reference for the in-depth study and development of antioxidant activity of of Coptis chinensis Franch.

关 键 词：川黄连 黄酮 抗氧化 1 1-二苯基-2-三硝基苯肼自由基 2 2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐阳离子自由基 

分 类 号：R284[医药卫生—中药学]