人参果主要病毒检测方法研究  

Study on the Detection Methods for Main Viruses of Ginseng Fruit

作　　者：裴怀弟[1] 宿兵兵 李玉斌 李淑洁[1] 王立光[1] 李琦 刘新星[1] PEI Huaidi;SU Bingbin;LI Yubin;LI Shujie;WANG Liguang;LI Qi;LIU Xinxing(Biotechnology Institute,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China;School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou Gansu 730070,China;Institute of Fruit and Floriculture Research,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China)

机构地区：[1]甘肃省农业科学院生物技术研究所,甘肃兰州730070 [2]兰州交通大学环境与市政工程学院,甘肃兰州730070 [3]甘肃省农业科学院林果花卉研究所,甘肃兰州730070

出　　处：《寒旱农业科学》2023年第4期365-368,共4页Journal of Cold-Arid Agricultural Sciences

基　　金：甘肃省农业科学院重点研发计划(2020GAAS13);甘肃省科协2020年学会助力精准扶贫项目(GXH20200820-1);甘肃省农业科学院成果转化项目(2021GAAS-CGZH05);兰州市科技计划项目(2019-1-67)。

摘　　要：为了给人参果病毒检验及抗病性研究提供支持,对人参果采用ELISA和RT-PCR两种检测方法对8份田间材料中的马铃薯M病毒(PVM)、番茄花叶病毒(ToMV)和烟草花叶病毒(TMV)进行检测,对比筛选病毒检测方法。结果表明,ELISA法检测PVM的阳性检出率为87.5%;ToMV为37.5%,疑似率为12.5%;TMV为37.5%。通过RT-PCR检测体系,从人参果样品中分别扩增出与试验设计大小相符的特异条带,PVM阳性检出率为100%,ToMV为50.0%,TMV为37.5%,检测灵敏性和准确性更高,检测结果符合率在87.5%以上。对扩增阳性产物进行凝胶回收测序,确定为目标条带。To provide references for virus testing and disease resistance research in ginseng fruit(Solanum muricatum Aiton),8 materials of ginseng fruit were tested from the fields by using ELISA and RT-PCR methods to detect potato virus M(PVM),tomato mosaic virus(ToMV)and tobacco mosaic virus(TMV),and comparison and screening of virus detection methods were conducted.The results showed that by using the ELISA method,the positive detection rates of PVM and ToMV were 87.5%and 37.5%,respectively,suspicion rate was 12.5%,and the positive rate of TVM was 37.5%.By using the RT-PCR detection system,specific bands were amplified from ginseng fruit samples which were consistent with the size of the test design,the positive detection rates of PVM,ToMV and TMV were 100%,50.0%and 37.5%,respectively,which indicated better detection sensitivity and accuracy,the coincident rate of the detected results was more than 87.5%.The amplified positive product was sequenced by gel recovery and was identified as the target band.

关 键 词：人参果 病毒 ELISA检测 RT-PCR检测 

分 类 号：S662.5[农业科学—果树学]