基于牛血清白蛋白增敏效应高灵敏检测强力霉素  

Highly Sensitive Detection of Doxycycline Based on Sensitization Effect of Bovine Serum Albumin

作　　者：王海波[1] 吴宁宁 陈林鸽 陶贝贝 WANG Haibo;WU Ningning;CHEN Linge;TAO Beibei(College of Chemistry and Chemical Engineering/Xinyang Key Laboratory of Functional Nanomaterials for Bioanalysis,Xinyang Normal University,Xinyang 464000,China)

机构地区：[1]信阳师范学院化学化工学院/信阳市功能纳米材料生物分析重点实验室,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2023年第2期196-199,共4页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(U1704153);河南省高层次人才特殊支持“中原千人计划”-中原青年拔尖人才(ZYQR201912153)。

摘　　要：基于牛血清白蛋白(BSA)对强力霉素(DC)的增敏效应,建立了一种简单、高灵敏度、高选择性的信号增强型荧光分析新方法以检测DC含量。由于增敏效应,BSA的存在显著增强了DC的荧光信号。这可能是由于DC分子进入BSA的疏水空腔结构中,DC分子的运动受到限制,导致DC的荧光信号显著增强。在优化的实验条件下,DC检测的线性范围为20 nmol/L~40μmol/L,检出限为5 nmol/L(R SN=3)。此外,该方法已经被用于牛奶样品中DC含量的测定。Based on the sensitization effect of bovine serum albumin(BSA)on doxycycline(DC),a simple,highly sensitive and selective turn-on fluorescence assay is developed for the detection of DC content.Due to the sensitization effect,the fluorescence signal of DC significantly enhances in the presence of BSA.It might be ascribe to that DC molecules could enter the hydrophobic cavity of BSA,and the DC rotation is restricted,leading to the fluorescence enhancement of DC.Under the optimized experimental conditions,the linear range of DC detection is 20 nmol/L~40μmol/L,and the limit of detection is 5 nmol/L(R SN=3).In addition,this method has been applied for the determination of DC in milk samples.

关 键 词：牛血清白蛋白 强力霉素检测 增敏效应 荧光分析法 

分 类 号：O657.39[理学—分析化学]