甘肃民乐紫皮大蒜茎尖脱毒技术研究  

Study on Virus-free Technology of Purple Garlic Stem Tip in Minle Gansu

作　　者：雷玉明 马金[4] 薛贵民 范宏伟 何振明[7] 张婧 Lei Yu-ming;Ma Jin;Xue Gui-min;Fan Hong-wei;He Zhen-ming;Zhang Jing(College of Agriculture and Biotechnology,Hexi University,Zhangye Gansu 734000;Qilian Mountain Pest Comprehensive Management Research Center of Hexi University,Zhangye Gansu 734000;Key Laboratory of Hexi Corridor Resourses Utilization of Gansu,Zhangye Gansu 734000;Gansu Advance Herding Technology Co,Ltd,Zhangye Gansu 734000;Gansu Dingfeng Potato Seed and Seedling Co.,Ltd,Minle Gansu 734500;Minle County Agricultural Technology Promotion Center,Minle Gansu 734500;Shandan County Agricultural Technology Popularization Center,Shandan Gansu 734100)

机构地区：[1]河西学院农业与生态工程学院,甘肃张掖734000 [2]河西学院祁连山有害生物综合治理研究中心,甘肃张掖734000 [3]河西走廊特色资源利用重点实验室,甘肃张掖734000 [4]甘肃前进生物科技发展有限公司,甘肃张掖734000 [5]甘肃鼎丰马铃薯种苗有限责任公司,甘肃民乐734500 [6]民乐县农业技术推广中心,甘肃民乐734500 [7]山丹县农业技术推广中心,甘肃山丹734100

出　　处：《河西学院学报》2023年第2期1-5,共5页Journal of Hexi University

基　　金：甘肃省农业生物技术研究与应用开发项目(项目编号:GNSW-2016-3)。

摘　　要：以甘肃民乐紫皮大蒜为试验材料,通过茎尖离体培养和激素配比试验,筛选最佳培养基,获得无病毒组培苗。结果表明,切取0.3~0.5mm+叶原基1片的茎尖外植体为标准,筛选出MS为茎尖基础启动培养基,成活率和成苗率均达90.0%以上;以MS+0.5mg/L~1.0mg/L6-BA+0.05mg/L~0.10mg/L NAA为增殖培养基,增殖倍数在3.11~4.25;以MS+NAA0.6mg/L为最适宜生根培养基,生根率最高达92%,茎尖脱毒率达94%,形成了甘肃民乐紫皮大蒜脱毒快繁关键技术体系。为提纯复壮地方特色品种提供了重要技术途径。Using Gansu Minle purple-skinned garlic as the test material,the best medium was screened by stem tip ex vi⁃vo culture and hormone ratio test to obtain virus-free histopathic seedlings.The results showed that MS was selected as the ba⁃sic starter medium for stem tips,and the survival rate and seedling establishment rate were above 90.0%;MS+0.5mg/L~1.0mg/L6-BA+0.05mg/L~0.10mg/L NAA was used as the proliferation medium,and the proliferation multiplier was between 3.11~4.25.The most suitable rooting medium was MS+NAA0.6mg/L.The rooting rate reached 92%,and the rate of stem tip detoxifica⁃tion reached 94%,forming a key technology system for detoxification and fast propagation of Gansu Minle purple garlic.It has provided an important technical way to purify and strengthen the local characteristic varieties.

关 键 词：民乐紫皮大蒜 茎尖培养 脱毒 

分 类 号：S633.4[农业科学—蔬菜学]