氮源水平对微藻深度脱氮除磷耦合生物大分子累积的影响及其细胞响应  

Effects of nitrogen source level on macromolecular accumulation coupled with nitrogen and phosphorus removal in microalgae and its cellular response

作　　者：丁婵 秦然 崔福义 赵志伟 梁志杰 DING Chan;QIN Ran;CUI Fuyi;ZHAO Zhiwei;LIANG Zhijie(College of Environment and Ecology,Chongqing University,Chongqing 400045,P.R.China)

机构地区：[1]重庆大学环境与生态学院,重庆400045

出　　处：《土木与环境工程学报（中英文）》2023年第3期183-195,共13页Journal of Civil and Environmental Engineering

基　　金：中央高校基本科研业务费(2021CDJJMRH-013);国家自然科学基金(51808066)。

摘　　要：比较普通小球藻、蛋白核小球藻和斜生栅藻在不同氮水平下的生长状况和污水深度脱氮除磷效能,并从生物大分子累积角度解析氮源水平对微藻脱氮除磷的影响机制,为实现污水深度脱氮除磷同时收获微藻生产能源以缓解能源危机提供理论依据。结果表明:硝氮为唯一氮源时,微藻生长状况和脱氮除磷效能明显优于以氨氮为唯一氮源的试验组,叶绿素a含量也高于氨氮组;各试验组中硝氮浓度越高,藻细胞数量越多,且小球藻细胞增长量明显高于斜生栅藻;7天内硝氮浓度≤8 mg/L时氮的去除率均能达到98%以上,但氮浓度低的试验组中叶绿素a含量低,这是因为氮是叶绿素合成的重要元素。微藻通过调节细胞内大分子物质含量来适应不同的生存环境,在营养受限制的条件下会消耗自身物质以满足生命活动的需要,氮限制条件会引起细胞内脂质积累,证实了氮源水平控制微藻污水深度脱氮除磷耦合生物大分子累积的可行性。The growth status of Chlorella vulgaris,Chlorella proteinosa and Scenedesmus obliquus under different nitrogen levels and the efficiency of advanced nitrogen and phosphorus removal in sewage were compared,and the effect mechanism of nitrogen source level on nitrogen and phosphorus removal in microalgae was analyzed from the perspective of biomacromolecule accumulation,and a theoretical basis was provided for advanced nitrogen and phosphorus removal in wastewater and harvesting microalgae to produce energy to alleviate energy crisis.The results showed that when nitrate is the only nitrogen source,the growth of microalgae and the efficiency of nitrogen and phosphorus removal were significantly better than that of the experimental group with ammonia nitrogen as the only nitrogen source,and the content of chlorophyll was also higher than that of the ammonia nitrogen group,because nitrogen is an important element of chlorophyll synthesis.The higher the nitrate concentration in each experimental group,the more the number of algae cells,and the growth of chlorella cells was significantly higher than that of Scenedesmus obliquus.Within seven days,the nitrogen removal rate was above 98%when nitrate concentration≤8 mg/L,but the chlorophyll-a content was low in the experimental group with low nitrogen concentration.Microalgae by adjusting the content of macromolecular substances in the cell to adapt to different living environment,under the condition of nutrition restricted consumes itself material to meet the needs of life activity,nitrogen limit conditions can lead to accumulation of lipid in cells,confirmed the possibility of nitrogen source level controlling on the depth of the wastewater biological nitrogen and phosphorus coupling feasibility of macromolecular accumulated.

关 键 词：微藻 污水脱氮 细胞响应 生物大分子累积 

分 类 号：X703.1[环境科学与工程—环境工程]