银翘散治疗儿童风热伤络型过敏性紫癜  

Yinqiao Powder in Treatment of Allergic Purpura with Wind-Heat Injured Collateral Syndrome in Children

作　　者：胡晓磊 熊维霖 王晴雯 宋纯东[2] 翟文生[2] 丁樱[2] 张霞[2] 朱庆军[1] 郭庆寅[2] HU Xiaolei;XIONG Weilin;WANG Qingwen;SONG Chundong;ZHAI Wensheng;DING Ying;ZHANG Xia;ZHU Qingjun;GUO Qingyin(Henan University of Chinese Medicine,Zhengzhou Henan China 450046;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou Henan China 450000)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南中医药大学第一附属医院,河南郑州450000

出　　处：《中医学报》2023年第5期905-914,共10页Acta Chinese Medicine

基　　金：河南省自然科学基金普通项目(212300410369);中国民族医药学会科研项目(2019KYXM-Z1106-48);河南省中医药科学研究专项普通课题项目(2019ZY2113)。

摘　　要：目的:采用16S rRNA和宏基因组测序方法,比较过敏性紫癜(henoch-sch9nlein purpura,HSP)患儿与健康儿童以及HSP患儿经银翘散治疗后口腔微生物群的组成差异,探究银翘散治疗风热伤络型HSP的可能机制。方法:共纳入初治HSP患儿20例,采集早晨700—800唾液标本为治疗前组,银翘散治疗2周后,再次采集同时段唾液为治疗后组,同时征集20例健康儿童作为健康对照组。采用Illumina NovaSeq测序平台对各组唾液微生物进行16S rRNA高通量测序,通过α多样性和β多样性分析,比较各组菌群物种组成和群落结构的差异,筛选出差异性菌群;采用宏基因组学比较HSP患儿与健康儿童功能基因差异。结果:与健康对照组相比,HSP治疗前组唾液菌群丰富度和多样性下降,但无显著性差异(P>0.05);与治疗前组比较,经银翘散治疗后组唾液菌群丰富度和多样性显著升高(P<0.05)。与健康对照组相比,治疗前组口腔菌群结构无明显差异(P>0.05);与治疗前组比较,治疗后组口腔菌群结构有显著差异(P<0.01)。使用线性判别分析(LDA)效应大小(LEfSe)和MetaStat方法分析,结果显示在门水平上,与健康对照组比较,治疗前组拟杆菌门(Bacteroidota)显著升高;与治疗前组比较,治疗后组Bacteroidota降低。与健康对照组比较,治疗前组放线菌门(Actinobacteriota)降低,与治疗前组比较,治疗后组Actinobacteriota明显升高。在属水平上,与健康对照组比较,治疗前组卟啉单胞菌属(Porphyromonas)、二氧化碳嗜纤维菌属(Capnocytophaga)和颗粒链球菌属(Granulicatella)升高,治疗后组显著低于治疗前组;与健康对照组比较,治疗前组韦荣氏球菌属(Veilonela)降低,治疗后组明显高于治疗前组。HSP患儿与健康儿童存在明显功能基因差异,HSP组患儿口腔细菌群落功能主要集中在信号转导、外源性生物降解和代谢、三大营养物质代谢、抗生素耐药等,而健康儿童口腔菌群与宿主维持生�Objective:To use 16SrRNA and metagenomic sequencing methods to compare the composition of oral microbiota between children with allergic purpura(henoch-sch nlein purpura,HSP)and healthy children,as well as HSP children treated with Yinqiao Powder,and to explore the possible mechanism of Yinqiao Powder in treatment of of Wind-Heat injured collateral HSP.Methods:A total of 20 children with newly diagnosed HSP were included.The saliva samples were collected from 7 GA6FA 00 to 8 GA6FA 00 in the morning as the pre-treatment group.After Yinqiao Powder treatment for 2 weeks,the saliva was collected again at the same time as the post-treatment group.20 cases were recruited at the same time.Healthy children served as a healthy control group.The Illumina NovaSeq sequencing platform was used to perform high-throughput sequencing of 16SrRNA in each group of saliva microorganisms.Throughα-diversity andβ-diversity analysis,the differences in the species composition and community structure of each group of flora were compared,and the differential flora was screened out;metagenomics was used for comparison Functional gene differences between children with HSP and healthy children.Results:Compared with the healthy control group,the richness and diversity of the salivary flora in the HSP treatment group decreased,but there was no significant difference(P>0.05);compared with the pre-treatment group,the salivary flora in the Yinqiao Powder treatment group Richness and diversity increased significantly(P<0.05).Compared with the healthy control group,there was no significant difference in the oral flora structure of the pre-treatment group(P>0.05);compared with the pre-treatment group,the oral flora structure of the post-treatment group was significantly different(P<0.01).Using the linear discriminant analysis(LDA)effect size(LEfSe)and the MetaStat method,it was shown that at the phylum level,compared with the healthy control group,the Bacteroidota in the pre-treatment group was significantly increased;compared with the pre-treatmen

关 键 词：过敏性紫癜 银翘散 风热伤络证 宏基因组学 16S rRNA 口腔微生态 唾液菌群 

分 类 号：R259.254[医药卫生—中西医结合]