不同天然群体子代家系苦豆子生物碱含量研究  

Study on Alkaloids Content of Sophora alopecuroides in Different Progenies of Natural Populations

作　　者：王瑗 李银祥[1,3] 史爱君 郝笑明 闫茂林 刘江[5] 刘矜杰 陈晓锋 WANG Yuan;LI Yinxiang;SHI Aijun;HAO Xiaoming;YAN Maolin;LIU Jiang;LIU Jinjie;CHEN Xiaofeng(Inner Mongolia Academy of Forestry Science,Hohhot 010010,China;Inner Mongolia Engineering Laboratory of Economic Forest Sterile Virus-free Cultivation,Hohhot 010010,China;Ordos Forest Ecosystem Research Station,Inner Mongolia,Ordos 016100,China;Inner Mongolia Daqingshan Nature Reserre Administration Hohhot Branch Huimin Qu Management station,Hohhot 010020,China;Inner Mongolia Lvtong Environmental Greening Limited Company,Hohhot 010010,China;West Ujimqin Banner Forestry Development Center,Inner Mongolia,Xilin Gol League 026200,China)

机构地区：[1]内蒙古自治区林业科学研究院,内蒙古呼和浩特010010 [2]经济林无菌脱毒培育内蒙古自治区工程实验室,内蒙古呼和浩特010010 [3]内蒙古鄂尔多斯森林生态系统国家定位观测研究站,内蒙古鄂尔多斯016100 [4]内蒙古自治区大青山自然保护区管理局呼和浩特分局回民区管理站,内蒙古呼和浩特010020 [5]内蒙古绿通环境绿化科技有限责任公司,内蒙古呼和浩特010010 [6]西乌珠穆沁旗林业事业发展中心,内蒙古锡林郭勒盟026200

出　　处：《内蒙古林业科技》2023年第1期17-22,共6页Journal of Inner Mongolia Forestry Science and Technology

基　　金：内蒙古自治区林业科学研究院技术推广与转化“科研能力提升项目”(104004001)。

摘　　要：采用高效液相色谱法(HPLC)对23个苦豆子天然群体子代家系植株地上部分(茎、叶)中的生物总碱以及5种生物碱单体(槐定碱、槐果碱、苦参碱、苦豆碱、氧化苦参碱)的含量进行了测定分析,结果显示:23个子代家系植株间的生物总碱及5种生物碱单体含量均有极显著差异(P<0.01);其中,生物总碱含量的均值为27.42 mg·g^(-1),变化范围为13.88~50.83 mg·g^(-1);5种生物碱单体的含量范围为0.0101~6.5932 mg·g^(-1),均值大小为:苦豆碱(3.6603 mg·g^(-1))>苦参碱(1.9303 mg·g^(-1))>氧化苦参碱(1.4124 mg·g^(-1))>槐果碱(0.2582 mg·g^(-1))>槐定碱(0.1278 mg·g^(-1));来自内蒙古乌海市(WH)的天然苦豆子群体子代家系中生物总碱以及苦豆碱等生物碱单体含量均较高。By use of high performance liquid chromatography,the content of the total alkaloids and five alkaloid monomers(sophoridine,sophocarpine,matrine,oxymatrine and aloperine)in the stems and leaves from different progenies of 23 natural populations of Sophora alopecuroides were measured and analyzed.The results showed that there were very significant differences in the content of the total alkaloids and five alkaloid monomers among different progenies from 23 natural populations.The content of the total alkaloids ranged from 13.88 mg·g^(-1) to 50.83 mg·g^(-1),with a mean value of 27.42 mg·g^(-1).The content of five alkaloid monomers ranged from 0.0101 mg·g^(-1) to 6.5932 mg·g^(-1),the order of the mean value was aloperine(3.6603 mg·g^(-1))>matrine(1.9303 mg·g^(-1))>oxymatrine(1.4124 mg·g^(-1))>sophocarpine(0.2582 mg·g^(-1))>sophoridine(0.1278 mg·g^(-1)).The content of the total alkaloids and alkaloid monomers of progeny of natural populations from Wuhai were higher than the others.

关 键 词：苦豆子 生物总碱 生物碱单体 含量差异 

分 类 号：S567[农业科学—中草药栽培]