基于液相色谱质谱联用技术的儿童免疫性血小板减少症血浆代谢组学研究  

Plasma Metabolomics of children immune thrombocytopenia based on Untargeted Liquid Chromatography–Mass Spectrometry

作　　者：徐文鑫[1] 肖慧婷 曾渊君 翁开枝 王尔莉 张春斌 XU Wen xin;XIAO Hui ting;ZENG Yuan jun;WENG Kai zhi;WANG er li;ZHANG Chun bin(Zhangzhou Health Vocational College,Zhangzhou 363000 China;Zhangzhou Affiliated Hospital of Fujian Medical University,Zhangzhou 363000 China)

机构地区：[1]漳州卫生职业学院医学技术系,福建漳州363000 [2]福建医科大学附属漳州市医院,福建漳州363000

出　　处：《黑龙江医药科学》2023年第2期40-45,共6页Heilongjiang Medicine and Pharmacy

基　　金：漳州市自然科技基金项目,编号:ZZ2018J38。

摘　　要：目的:基于LC-MS寻找儿童ITP潜在生物标记物。方法:采集患儿及健康儿童外周血进行LC-MS检测,通过统计学方法分析,经数据库鉴定代谢物结构。结果:LC-MS/MS检测获得原始数据,经主成分分析、层次聚类分析、秩和检验及HMDB数据库中匹配鉴定,再通过ROC曲线分析,最终筛选鉴定出5种潜在生物标志物:间甲酚、腐胺、5-羟色胺、2-氧代精氨酸、精氨基琥珀酸。结论:检测鉴定出的5种关键差异性代谢物可能可以作为鉴别ITP患儿和健康儿童的临床实验室生物标记物。Objective:To find the potential biomarkers of ITP based on LC-MS.Methods:The peripheral blood of the patient and healthy children was collected for LC-MS detection,and the metabolite structure was identified by database.Results:The original data obtained by LC-MS/MS were identified by principal component analysis,hierarchical cluster analysis,rank sum test and HMDB database matching,and then by ROC curve analysis,5 potential biomarkers were finally screened and identified:m-cresol,putreamine,5-hydroxytryptamine,2-oxo-arginine,argininosuccinic acid.Conclusionl:The five key differential metabolites identified may be used as clinical laboratory biomarkers to distinguish ITP children from healthy children.

关 键 词：儿童免疫性血小板减少症 代谢组学 液相色谱-质谱联用技术 

分 类 号：R558.2[医药卫生—血液循环系统疾病]