基质辅助激光解吸电离飞行时间质谱检测肠杆菌科细菌产碳青霉烯酶的应用价值  

Application value of matrix-assisted laser desorption/ionization time of flight mass spectrometry in detection of carbapenemase-producing Enterobacteriaceae

作　　者：吴灿权[1] 刘绮明 仇绮琳 郑悦康[1] 区金结 WU Can-quan;LIU Qi-ming;QIU Qi-lin;ZHENG Yue-kang;OU Jin-jie(Zhongshan Center for Disease Control and Prevention,Zhongshan,Guangdong 528400,China)

机构地区：[1]中山市疾病预防控制中心病原微生物与生物检验所,广东中山528400

出　　处：《中华医院感染学杂志》2023年第5期663-666,共4页Chinese Journal of Nosocomiology

基　　金：中山市社会公益与基础研究基金资助项目(2022B1034)。

摘　　要：目的评估基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)检测肠杆菌科细菌产碳青霉烯酶的应用价值。方法选取2018-2021年中山市致病菌识别网分离的111株肠杆菌科细菌。所有菌株采用纸片扩散法(K-B法)、改良碳青霉烯灭活试验(mCIM)、MALDI-TOF MS进行检测,分析评估MALDI-TOF MS在检测肠杆菌科细菌产碳青霉烯酶的能力。结果111株菌株采用mCIM和MALDI-TOF MS两种方法进行检测,均检出32株菌产碳青霉烯酶,其余79株菌不产酶。结论mCIM和MALDI-TOF MS两种方法对肠杆菌科细菌产碳青霉烯酶检测结果一致,具有相同的灵敏度,且MALDI-TOF MS检测时间大幅减少,可实现批量检测,具有推广应用的价值。OBJECTIVE To evaluate the application value of matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS)in detection of carbapenemase-producing Enterobacteriaceae.METHODS Totally 111 strains of Enterobacteriaceae that were isolated from Zhongshan pathogen identification network between 2018 and 2021.All of the strains were detected by means of disk diffusion method(K-B method),modified carbapenem inactivation test(mCIM)and MALDI-TOF-MS.The capability of MALDI-TOF MS in detection of carbapenemase-producing Enterobacteriaceae was observed.RESULTS mCIM and MALDI-TOF MS were employed to detect 111 strains,both methods detected 32 strains of carbapenemase-producing Enterobacteriaceae,and the rest of 79 strains did not produced enzyme.CONCLUSION The detection results of mCIM and MALDI-TOF MS are the same,and they have the same sensitivity.MALDI-TOF MS requires significantly less detection time enables batch detections,and it is worthy to be promoted.

关 键 词：基质辅助激光解吸电离飞行时间质谱 改良碳青霉烯灭活试验 碳青霉烯酶 肠杆菌科细菌 耐药性 抗菌药物 

分 类 号：R378.2[医药卫生—病原生物学]