生殖道支原体感染与胎膜早破的关系及其对线粒体凋亡通路的影响  

Association of genital tract mycoplasma infection with premature rupture of membranes and its influence on mitochondrial apoptosis pathway

作　　者：杨园园 李婷 阎晓丽 薛莎 李芳 YANG Yuan-yuan;LI Ting;YAN Xiao-li;XUE Sha;LI Fang(Yuncheng Nursing Vocational College,Yuncheng,Shanci 044000,China;不详)

机构地区：[1]运城护理职业学院微免教研室,山西运城044000 [2]运城市中心医院妇科,山西运城044000 [3]运城护理职业学院妇产科教研室,山西运城044000

出　　处：《中华医院感染学杂志》2023年第6期910-914,共5页Chinese Journal of Nosocomiology

基　　金：山西省教育科学“十三五”规划教育政策研究专项课题基金资助项目(ZC-20072)。

摘　　要：目的 探讨生殖道支原体感染与胎膜早破(PROM)的关系及其对线粒体凋亡通路的影响。方法 选取2019年12月-2021年12月于运城市中心医院分娩伴有生殖道支原体感染产妇121例为感染组和同期无生殖道感染产妇118名为健康组,比较两组产妇PROM情况和妊娠结局,感染组产妇根据是否发生PROM分为感染PROM组45例和感染非PROM组76例,酶联免疫吸附试验检测胎膜样本半胱氨酸蛋白酶(Caspase)-3、Bcl相关X蛋白(Bax)、B细胞淋巴瘤/白血病-2(Bcl-2)水平,比较分析两组产妇胎膜组织Caspase-3、Bax和Bcl-2水平差异,受试者工作特征(ROC)曲线分析胎膜组织Caspase-3、Bax和Bcl-2水平对生殖道支原体感染并PROM预测价值。结果 感染组产妇PROM、早产、新生儿肺炎、病理性黄疸占比均高于健康组(P<0.05)。感染组产妇胎膜组织Caspase-3、Bax水平高于健康组(P<0.05),Bcl-2水平低于健康组(P<0.05)。感染PROM组产妇胎膜组织Caspase-3、Bax水平高于感染非PROM组(P<0.05),Bcl-2水平低于感染非PROM组(P<0.05)。Caspase-3、Bax、Bcl-2水平及其联合检测时的曲线下面积(AUC)分别为0.706、0.741、0.768和0.914,且联合检测时的AUC高于其单独检测(P<0.05)。结论 生殖道支原体感染增加产妇PROM和新生儿不良结局风险,可能与激活胎膜组织中线粒体依赖性细胞凋亡通路有关。OBJECTIVE To explore the relationship between genital tract mycoplasma infection and premature rupture of membrane(PROM) and observe its influence on mitochondrial apoptosis pathway. METHODS A total of 121 puerpera with genital tract mycoplasma infection and 118 puerpera without genital tract infection who gave birth in Yuncheng Nursing Vocational College from Dec 2019 to Dec 2021 were recruited as the research subjects and were respectively assigned as the infection group and the healthy group. The incidence of PROM and pregnancy outcomes were compared between the two groups. The puerpera of the infection group were divided into the PROM group with 45 cases and the non-PROM group with 76 cases according to the status of PROM. The levels of cysteine proteinase-3(Caspase-3), Bcl-2 associated X protein(Bax) and B-cell lymphoma/leukemia-2(Bcl-2) in fetal membrane samples were detected by means of enzyme-linked immunosorbent assay. The levels of fetal membrane tissue Caspase-3, Bax and Bcl-2 were compared between the two groups, and the values of fetal membrane tissue Caspase-3, Bax and Bcl-2 in prediction of genital tract mycoplasma infection and PROM were analyzed by receiver operating characteristic(ROC) curve. RESULTS The proportion of the puerpera with PROM, preterm delivery, neonatal pneumonia and pathologic jaundice were higher in the infection group than in the healthy group(P<0.05). The levels of fetal membrane tissue Caspase-3 and Bax of the infection group were higher than those of the healthy group(P<0.05). The levels of fetal membrane tissue Caspase-3 and Bax of the PROM group were higher than those of the non-PROM group(P<0.05), the Bcl-2 level of the PROM group was lower than that of the non-PROM group(P<0.05). The area under curves(AUC) of Caspase-3, Bax, Bcl-2 and the joint detection were respectively 0.706, 0.741, 0.768 and 0.914, and the AUC of the joint detection was larger than that of the single detection(P<0.05). CONCLUSION The genital tract mycoplasma infection may increase the risk of PROM an

关 键 词：胎膜早破 生殖道 支原体感染 细胞凋亡 线粒体途径 

分 类 号：R714.433[医药卫生—妇产科学]