基于UPLC-Q-TOF-MS的速效救心丸化学成分定性分析  

Analysis of chemical constituents of Suxiao Jiuxin Pills by UPLC-Q-TOF-MS

作　　者：贾志鑫 丛诗语 潘明霞 刘力榕 刘洁 李月婷[3] 肖红斌[1,2] JIA Zhixin;CONG Shiyu;PAN Mingxia;LIU Lirong;LIU Jie;LI Yueting;XIAO Hongbin(Beijing Research Institute of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;Research Center of Chinese Medicine Analysis and Transformation,Beijing University of Chinese Medicine,Beijing 100029,China;School of Chinese Materia Medical,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学北京中医药研究院,北京100029 [2]北京中医药大学中药分析与转化研究中心,北京100029 [3]北京中医药大学中药学院,北京100029

出　　处：《药物评价研究》2023年第2期330-341,共12页Drug Evaluation Research

基　　金：北京市自然科学基金(7214284);国家自然科学基金(82173957,81903766)。

摘　　要：目的采用超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF-MS)对速效救心丸的化学成分进行研究。方法采用Agilent Poroshell 120 EC C18柱(150 mm×3.0 mm,2.7μm),以0.1%甲酸水-乙腈为流动相梯度洗脱,体积流量为0.8 mL·min^(-1)(分流比1∶2);采用Dual AJS ESI源在正、负离子模式下分别采集数据。构建化合物库,根据化合物精确相对分子质量及二级碎片离子信息结合参考文献数据,鉴定速效救心丸的主要化学成分。结果从速效救心丸中分离和鉴定出90个化学成分,其中苯酞类化合物47个,有机酸类化合物11个,生物碱类化合物13个,萜类化合物4个,其他类化合物15个。结论该方法快速、准确、便捷,为阐明速效救心丸的药效物质基础及进一步研究其体内代谢过程提供了科学的数据支撑和方法参考。Objective The chemical constituents of Suxiao Jiuxin Pills(SJP)were studied by ultra-high-performance liquid chromatography coupled with quadruple time of flight mass spectrometry(UPLC-Q-TOF-MS).Methods Agilent Poroshell 120 EC C18 column(150 mm×3.0 mm,2.7μm)was used for gradient elution with 0.1%formic acid aqueous solution and acetonitrile as mobile phases.The volume flow rate was 0.8 mL·min^(-1)(split ratio 1∶2).Dual AJS ESI source was used to collect data in positive and negative ion modes.A compound library was established.According to the precise relative molecular weight and secondary fragment ion information of compounds,the main chemical constituents of SJP were identified with reference data.Results 90constituents were identified from SJP,including 47 phthalides,11 organic acids,13 alkaloids,four terpenoids and 15 other compounds.Conclusion The method is rapid,accurate and convenient,which provides scientific data support and method reference for elucidating the pharmacodynamic substance basis of SJP and further studying its metabolism process in vivo.

关 键 词：速效救心丸 UPLC-Q-TOF-MS 化学成分 苯酞类 有机酸类 生物碱类 

分 类 号：R284.1[医药卫生—中药学]