大果木姜子精油及浸膏对心肌损伤大鼠的保护作用  

Protective effect of essential oil and extract from Cinnamomum migao based on myocardial injury in rats

作　　者：王健 魏晴 徐文芬 刘育辰 李蒙禹 杨烨 孙庆文 WANG Jian;WEI Qing;XU Wenfen;LIU Yuchen;LI Mengyu;YANG Ye;SUN Qingwen(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Guizhou Provincial Key Laboratory of Pharmacognosy of Traditional Chinese Medicine,Guiyang 550025,China;不详)

机构地区：[1]贵州中医药大学药学院,贵阳550025 [2]贵州省中药生药学重点实验室,贵阳550025

出　　处：《实用医学杂志》2023年第7期804-812,共9页The Journal of Practical Medicine

基　　金：国家自然科学基金委-贵州省人民政府联合基金项目子课题(编号:U1812403-2)。

摘　　要：目的探讨大果木姜子精油、浸膏对心肌损伤大鼠的保护作用。方法将SD大鼠随机分为正常对照组(CG)、模型组(MG)、复方丹参组(FFDS)、尼可地尔组(Nic),大果木姜子精油低剂量组(GO-1)、精油高剂量组(GO-2),浸膏低剂量组(GE-1)、浸膏高剂量组(GE-2),每组11只。除CG外,各组大鼠冰水力竭游泳14 d,第12天后于颈部皮下多点注射异丙肾上腺素(sisoprotereno,ISO)50 mg/kg,连续3 d,建立心肌缺血损伤模型。造模后灌胃相应药物,每天2次,连续7 d。采集大鼠治疗前后Ⅱ导联心电图,观察治疗后HE、Masson着染的腹主动脉、心肌组织病理变化,试剂盒检测心肌标志物门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)、肌钙蛋白Ⅰ(cTn-Ⅰ),炎性因子白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α),和超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果造模大鼠Ⅱ导联心电图表现主要为室上性心律失常,心肌组织出现炎性细胞浸润,细胞间质发生胶质沉积,说明大鼠心肌损伤模型构建成功。与CG相比,MG大鼠心电ST段抬高,伴有心律失常,心肌组织有大量细胞坏死和炎性细胞浸润,可见胶原纤维和结缔组织增生;血清中LDH、AST活性及CK-MB、cTn-Ⅰ、IL-6、TNF-α含量显著增高(P<0.01),SOD活性降低(P<0.05),MDA表达量显著增高(P<0.05)。与MG比较,FFDS、Nic及GO-1、GO-2,GE-1、GE-2均可明显改善心肌组织、腹主动脉病理损伤及心电变化。相对于GE-1、2,GO-1更能抑制cTn-Ⅰ、MDA的表达,GO-2更能抑制AST、IL-6的水平;相较于GO-1、2,GE-1更能降低CK-MB、TNF-α的含量,GE-2更能降低LDH活性,提高SOD活力。结论大果木姜子精油、浸膏均能通过减轻氧化应激损伤、抑制心肌损伤标志物表达及炎症反应来修复损伤心肌组织,浸膏部位在降低LDH、CK-MB、TNF-α表达及提高SOD活性方面更具优势。Objective To investigate the protective effects of essential oil and extract of Cinnamomum migao on myocardial injury in rats.Methods SD rats were randomly divided into normal control group(CG),model group(MG),compound salvia miltiorrhiza group(FFDS),nicorandil group(Nic),low dose essential oil group(GO⁃1),high dose essential oil group(GO⁃2),low dose extract group(GE⁃1)and high dose extract group(GE⁃2),with 11 rats in each group.Rats in each group,except those in CG,swam exhaustively with ice water for 14 days,and ISO(50 mg/kg)was injected subcutaneously at multiple points in the neck on the 12th day for consecutive 3 days to establish a myocardial ischemia injury model.After model establishment,the corresponding drugs were intragastrically administered twice a day for 7 days.LeadⅡECG was collected from the rats before and after treatment to observe the pathological changes of abdominal aorta and myocardium stained with HE and Masson after treatment.Myocardial markers AST,LDH,CK⁃MB,cTn⁃I,inflammatory cytokines IL⁃6,TNF⁃α,SOD activity and MDA content were measured by kits.Results The main ECG findings of leadⅡin the model rats were supraventricular arrhythmia,inflammatory cell infiltration in myocardial tissue and glial deposition in interstitial cells,indicating that the rat myocardial injury model was successfully constructed.Compared with those in CG,rats in MG had ST segment elevation,accompanied by arrhythmia,a large number of cell necrosis and inflammatory cell infiltration in myocardial tissue,and collagen fiber and connective tissue proliferation were observed;LDH and AST activities and CK⁃MB,cTn⁃1,IL⁃6,and TNF⁃αcontents in serum were significantly higher(P<0.01);SOD activity was decreased(P<0.05),and MDA expression was significantly higher(P<0.05).Compared with those in MG,pathological damage of myocardial tissue,abdominal aorta and ECG changes were significantly improved in FFDS,Nic,GO⁃1,GO⁃2,GE⁃1 and GE⁃2.Compared with that in GE⁃1 and GE⁃1,the expression of cTn

关 键 词：大果木姜子 心肌损伤 精油 非挥发油 主动脉 

分 类 号：R29[医药卫生—民族医学]