N-乙酰半胱氨酸对支气管哮喘大鼠CXCL8-CXCR1/2及TRPV1神经元敏感性的影响  

Effects of N-acetylcysteine on the sensitivity of CXCL8-CXCR1/2 and TRPV1 neurons in bronchial asthmatic rats

作　　者：王萍 农开旭 邢春蕊 WANG Ping;NONG Kai-Xu;XING Chun-Rui(Haikou People's Hospital,Haikou 570208,Hainan,China)

机构地区：[1]海口市人民医院,海南海口570208

出　　处：《中国老年学杂志》2023年第9期2176-2181,共6页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81860840)。

摘　　要：目的探究N-乙酰半胱氨酸对支气管哮喘大鼠CXC趋化因子配体(CXCL)8-CXC趋化因子受体(CXCR)1/2及瞬时受体电位通道蛋白(TRP)V1神经元敏感性的影响。方法选取80只SPF级SD雄性大鼠,随机分为正常(NO)组、模型(MO)组、N-乙酰半胱氨酸(NAC)组、急支糖浆(ES)组,每组20只,对MO组、NAC组、ES组进行支气管哮喘建模,建模成功后,NAC组、ES组每天分别于腹腔内注射2 ml N-乙酰半胱氨酸注射液、急支糖浆灌胃10 g/kg剂量,NO组、MO组同期灌胃同体积生理盐水,通过苏木素-伊红(HE)染色法检测肺组织病理形态、酶联免疫吸附试验(ELISA)、实时荧光定量聚合酶链反应(RT-PCR)、Western印迹检测血清及肺组织中CXCL8、CXCR1、CXCR2、TRPV1含量、mRNA及蛋白表达,并分析N-乙酰半胱氨酸对支气管哮喘大鼠CXCL8-CXCR1/2及TRPV1神经元敏感性。结果与NO组相比,MO组咳嗽次数明显增加(P<0.05),而NAC组、ES组与MO组相比,其咳嗽次数明显降低(P<0.05),且NAC组比ES组降低明显(P<0.05);与NO组相比,MO组细支气管管腔、肺泡腔内可见渗出液、脱落的上皮细胞等,远端肺泡可见局部肺不张及周围肺大泡,且肺间质明显增厚,炎性细胞浸润明显,而与MO组比较,ES组、NAC组症状明显减少,部分肺间质的组织结构趋向正常,部分肺泡轻度扩张,且NAC组比ES组明显降低(P<0.05);与NO组对比,MO组CXCL8、CXCR1、CXCR2、TRPV1含量、mRNA及蛋白表达均明显升高(P<0.05),NAC组、ES组与MO组相比均显著降低(P<0.05),且NAC组比ES组明显降低(P<0.05)。结论N-乙酰半胱氨酸可以显著降低咳嗽次数,减少支气管哮喘症状,可使CXCL8-CXCR1/2及TRPV1神经元敏感性显著降低。Objective To explore the effect of N-acetylcysteine on the sensitivity of CXC chemokine ligand(CXCL)8-CXC chemokine receptor(CXCR)1/2 and transient receptor potential channel protein(TRP)V1 neurons in bronchial asthmatic rats.Methods 80 SPF male SD rats were selected and randomly divided into normal(NO)group,model(MO)group,N-acetylcysteine(NAC)group,emergency branch syrup(ES)group,with 20 rats in each group.The bronchial asthma modeling for the MO group,NAC group and ES group were performed.After the modeling was successful,the NAC group and the ES group were injected intraperitoneally with 2 ml of N-acetylcysteine injection and emergency branch syrup intragastric administration with 10 g/kg dose every day,NO group and MO group were given the same volume of normal saline intragastric administration at the same time,the pathological form of lung tissue was detected by htoxylin eosin(HE)staining method,enzyme linked immunosorbent assay(ELISA),real-time fluorescence quantitative polymerase chain reaction(RT-PCR)and Western blot were used to detect the contents,mRNA and protein expressions of CXCL8,CXCR1,CXCR2 and TRPV1 in serum and lung tissues,and the sensitivity of N-acetylcysteine to CXCL8-CXCR1/2 and TRPV1 neurons in bronchial asthmatic rats were analyzed.Results Compared with the NO group,the number of coughs in the MO group was significantly increased(P<0.05),while the number of coughs in the NAC group and the ES group were significantly reduced than those in MO group(P<0.05),and that of the NAC group was significantly reduced than that in the ES group(P<0.05);compared with the NO group,exudates and exfoliated epithelial cells were seen in the bronchiole lumen and alveolar cavity of the MO group,and local atelectasis and surrounding areas were seen in the distal alveoli lung bullae,and the lung interstitium was obviously thickened,and the inflammatory cell infiltration was obvious.Compared with the MO group,the symptoms of the ES group and the NAC group were significantly reduced,the tissue structure of some l

关 键 词：N-乙酰半胱氨酸 支气管哮喘 CXC趋化因子配体(CXCL)8 CXC趋化因子受体(CXCR)1 CXCR2 瞬时受体电位通道蛋白(TRP)V1 

分 类 号：R963[医药卫生—微生物与生化药学]