紫草素对乳腺恶性肿瘤细胞凋亡及PI3K/AKT信号通路的影响  

Effects of Alkannin on Apoptosis and PI3K/Akt Signaling Pathway in Breast Cancer Cells

作　　者：赖瑞 史晓光[1] 左禧萌 汪唐顺[1] 刘洁丽 杨臻瑞 冯雪[1] 王玉坤 高爽 孙萍 LAI Rui;SHI Xiaoguang;ZUO Ximeng;WANG Tangshun;LIU Jieli;YANG Zhenrui;FENG Xue;WANG Yukun;GAO Shuang;SUN Ping(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100010,China)

机构地区：[1]北京中医药大学东直门医院,北京100010

出　　处：《世界中医药》2023年第6期788-791,共4页World Chinese Medicine

基　　金：湖北省陈孝平科技发展基金项目(CXPJJH12000002-2020010)——三阴性乳腺癌病人术后应用槐耳颗粒的疗效及机制研究。

摘　　要：目的:探讨紫草素对乳腺恶性肿瘤MDA-MB-231和MCF-7细胞凋亡以及PI3K/AKT信号通路表达的影响。方法:将MDA-MB-231及MCF-7细胞系用含不同浓度紫草素的完全培养基(分组为0、0.2、0.4、0.8、1.0 mg/mL)进行培养,CCK-8法检测细胞活性,Annexin V-FTIC/PI染色后流式细胞仪检测细胞凋亡情况,蛋白质印迹法测定细胞内PI3K/AKT表达情况。结果:与空白对照组(0 mg/mL)比较,紫草素浓度为0.8 mg/mL以上时对乳腺癌MDA-MB-231及MCF-7细胞生长具有显著抑制作用,最佳作用浓度为1.0 mg/mL;与空白对照组比较,MDA-MB-231及MCF-7细胞的凋亡水平随紫草素浓度增加而增加,且差异有统计学意义(P<0.05);与空白对照组比较,经紫草素处理的MDA-MB-231及MCF-7细胞PI3K表达均出现不同程度下降,差异有统计学意义(P<0.05)。结论:紫草素具有针对MDA-MB-231及MCF-7乳腺癌细胞系的抗肿瘤作用,其机制可能与其抑制PI3K/AKT信号通路有关。Objective:To investigate the effects of alkannin on the apoptosis and phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt)signaling pathway in malignant breast cell lines MDA-MB-231 and MCF-7.Methods:MDA-MB-231 and MCF-7 cells were cultured in the complete media containing different concentrations of alkannin(0,0.2,0.4,0.8,and 1.0 mg/mL).The cell viability,apoptosis,and PI3K/Akt expression were determined by CCK-8 assay,Annexin V-FTIC/PI staining-flow cytometry,and western blotting,respectively.Results:Compared with the blank control group(0 mg/mL),alkannin at a concentration of 0.8 mg/mL and higher significantly inhibited the growth of MDA-MB-231 and MCF-7 cells,and that of 1.0 mg/L demonstrated the best performance.The apoptosis of MDA-MB-231 and MCF-7 cells increased with the rise in alkannin concentration(P<0.05).Compared with the blank control group,alkannin down-regulated the expression of PI3K in MDA-MB-231 and MCF-7 cells(P<0.05).Conclusion:Alkannin may exert the anti-tumor effects on MDA-MB-231 and MCF-7 cells by inhibiting the PI3K/Akt signaling pathway.

关 键 词：紫草素 乳腺恶性肿瘤 细胞凋亡 高转移恶性乳腺癌细胞系 原位雌激素受体阳性乳腺癌细胞系 磷脂酰肌醇3激酶 

分 类 号：R284[医药卫生—中药学] R273[医药卫生—中医学]