OxyR介导的细菌氧化胁迫应答调控机制研究进展  

Research progress in OxyR-mediated regulation of oxidative stress in bacteria

作　　者：洪晓丽 杨倩雯 音建华 万芬 HONG Xiaoli;YANG Qianwen;YIN Jianhua;WAN Fen(School of Laboratory Medicine and Bioengineering,Hangzhou Medical College,Hangzhou 310053,Zhejiang,China;College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou 310014,Zhejiang,China)

机构地区：[1]杭州医学院检验医学院/生物工程学院,浙江杭州310053 [2]浙江工业大学生物工程学院,浙江杭州310014

出　　处：《微生物学报》2023年第4期1392-1403,共12页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31900021,32270044)。

摘　　要：OxyR属于LysR型转录因子家族的氧化胁迫调控蛋白,是细菌抵抗氧化胁迫压力的重要调控因子。OxyR能够通过调控过氧化氢酶和过氧化物酶等抗氧化基因的表达清除H_(2)O_(2)、参与铁代谢控制胞内过氧化物的产生以及修复生物大分子氧化损伤,从而抵抗氧化胁迫。OxyR的基因表达调控功能依赖于其还原态和氧化态之间的转变,改变调控蛋白对下游基因调控区的亲和能力。氧化态OxyR识别启动子区的结合序列,激活或抑制过氧化氢酶等基因的表达。还原态和氧化态的转换依赖于在氧化状态下分子间二硫键的形成。本文综述了近年来细菌OxyR调控基因表达的最新研究进展,有助于深入理解OxyR在细菌抵抗氧化胁迫的作用方式,为相关致病菌的防治奠定分子基础。OxyR,an oxidative stress regulator of the LysR family,is essential for bacteria to survive when confronting the threat of hydrogen peroxide(H_(2)O_(2)).OxyR regulates the expression of multiple genes such as those encoding catalase and peroxidase to scavenge H_(2)O_(2),participates in the iron metabolism to control the generation of peroxides,and repairs biomacromolecule damage.Functioning as a two-state redox switch depending on the formation of intracellular disulfide bond in the oxidizing and reducing environments,OxyR could recognize specific binding motif of target genes and activate/repress their expression.Here,we review the latest research progress in the OxyR-mediated regulation of gene expression in bacteria,which will help to further understand the role of OxyR in resisting oxidative stress and provide a molecular basis for preventing and controlling related pathogens.

关 键 词：氧化胁迫 OXYR 过氧化氢酶 基因表达调控 

分 类 号：Q933[生物学—微生物学]