紫草素通过上调微小RNA-637调控肝癌细胞的增殖、迁移和侵袭  

Shikonin regulates the proliferation, migration and invasion of liver cancer cells by up-regulating microRNA-637

作　　者：高志强[1] 李超博 李丁洋 刘兆臣 葛鹏磊 Gao Zhiqiang;Li Chaobo;Li Dingyang;Liu Zhaochen;Ge Penglei(Department of Hepatic and Biliary Pancreatic Surgery,Henan Key Laboratory of Digestive Organ Transplantation,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院肝胆胰外科、河南省消化器官移植重点实验室,郑州450052

出　　处：《中华实验外科杂志》2023年第2期247-250,共4页Chinese Journal of Experimental Surgery

基　　金：河南省医学教育研究项目(Wjlx2020052)。

摘　　要：目的探讨紫草素对肝癌Huh-7细胞增殖、迁移和侵袭的影响及其可能的分子机制。方法实时荧光定量聚合酶链反应(FQ-PCR)检测微小RNA(miR)-637的表达水平;噻唑蓝(MTT)检测Huh-7细胞增殖;Transwell实验检测Huh-7细胞的迁移和侵袭;蛋白质印迹法检测Huh-7细胞中增殖、迁移和侵袭相关蛋白的表达。两组间比较采用t检验,多组件比较采用单因素方差分析,组内间比较采用SNK-q检验。结果肝癌组织中miR-637表达水平低于癌旁组织(0.43±0.05比1.00±0.11,t=25.840,P<0.05);1、2、3、4μg/ml紫草素组Huh-7细胞存活率低于对照组(82.24±7.82、54.15±6.16、43.34±6.35、38.68±5.24比100.00±9.69,t=4.279、11.980、14.670、16.700,P<0.05);2μg/ml紫草素处理组Huh-7细胞的增殖(0.81±0.07比1.46±0.18,t=10.097,P<0.05)、迁移(79.28±8.12比137.19±14.36,t=10.531,P<0.05)和侵袭(48.67±5.32比102.52±11.25,t=12.982,P<0.05)能力低于对照组。过表达miR-637组Huh-7细胞的增殖(0.78±0.08比1.52±0.13,F=96.865,P<0.05)、迁移(74.12±8.16比134.26±15.69,F=77.227,P<0.05)和侵袭(74.12±8.16比134.26±15.69,F=131.214,P<0.05)能力低于miR-NC组。2μg/ml紫草素+抗miR-637组Huh-7细胞增殖(1.27±0.15比0.75±0.08,F=65.743,P<0.05)、迁移(116.28±12.53比71.45±7.82,F=58.264,P<0.05)和侵袭(86.41±9.23比46.32±4.92,F=113.920,P<0.05)能力高于2μg/ml紫草素+抗miR-NC组。结论紫草素可通过上调miR-637抑制Huh-7细胞的增殖、迁移和侵袭。Objective To investigate the effects and possible molecular mechanism of shikonin on the proliferation,migration and invasion of liver cancer.Methods Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression level of miR-637.MTT and Transwell assays were used to detect Huh-7 cell proliferation,migration and invasion.Western blotting was used to detect the expression of proliferation,migration and invasion-related proteins in Huh-7 cells.The comparison between the two groups was done by t test,the multi-component comparison by one-way analysis of variance,and the comparison between groups by the SNK-q test.Results Compared with adjacent tissues,the expression levels of miR-637 in liver cancer tissues were decreased(0.43±0.05 vs.1.00±0.11,t=25.840,P<0.05).As compared with the control group,1,2,3,4μg/ml shikonin significantly reduced Huh-7 cell viability(82.24±7.82,54.15±6.16,43.34±6.35,38.68±5.24 vs.100.00±9.69,t=4.279,11.980,14.670,16.700,P<0.05).The proliferation(0.81±0.07 vs.1.46±0.18,t=10.097,P<0.05),migration(79.28±8.12 vs.137.19±14.36,t=10.531,P<0.05)and invasion(48.67±5.32 vs.102.52±11.25,t=12.982,P<0.05)of Huh-7 cells in 2μg/ml shikonin treatment group were lower than those in control group.The proliferation(0.78±0.08 vs.1.52±0.13,F=96.865,P<0.05),migration(74.12±8.16 vs.134.26±15.69,F=77.227,P<0.05)and invasion(74.12±8.16 vs.134.26±15.69,F=131.214,P<0.05)of Huh-7 cells in miR-637 overexpression group were lower than those in miR-NC group.The proliferation(1.27±0.15 vs.0.75±0.08,F=65.743,P<0.05),migration(116.28±12.53 vs.71.45±7.82,F=58.264,P<0.05)and invasion(86.41±9.23 vs.46.32±4.92,F=113.920,P<0.05)of Huh-7 cells in 2μg/ml shikotin+anti-miR-637 group were higher than those in 2μg/ml shikonin+anti-miR-NC group.Conclusion Shikonin inhibited the proliferation,migration and invasion of Huh-7 cells by up-regulating miR-637.

关 键 词：肝癌 紫草素 微小RNA 增殖 迁移 侵袭 

分 类 号：R285[医药卫生—中药学]