应用实时荧光PCR法建立献血者血小板特异性抗原HPA-1~6/10/15/21基因分型资料库  

Establishment of platelet donor gene bank involving specific antigen HPA 1-6/10/15/21 by real-time fluorescent PCR

作　　者：袁文声 陈前英 林惠燕 方育如 郑晓兰 邝永晴 YUAN Wensheng;CHEN Qianying;LIN Huiyan;FANG Yuru;ZHENG Xiaolan;KUANG Yongqing(Zhongshan Central Blood Station,Zhongshan 528400,China)

机构地区：[1]中山市中心血站,广东中山528400

出　　处：《中国输血杂志》2023年第3期204-208,共5页Chinese Journal of Blood Transfusion

基　　金：中山市社会公益与基础研究项目(2021B1123)。

摘　　要：目的研究中山地区单采血小板献血者人类血小板特异性抗原HPA-1~6/10/15/21共9对抗原基因多态性区域分布特点,建立中山地区血小板献血者HPA基因分型资料库。方法用实时荧光定量PCR-TaqMan探针法对中山地区192名单采血小板献血者进行HPA-1~6/10/15/21基因分型,计算基因型频率、基因频率。结果HPA-1~6/10/15/21中,HPA-4、10系统呈单特异性,只检测出aa基因型,未检出等位基因HPA-b;HPA-1、2、5、6、21基因型以aa占绝大多数;HPA-3、15具有较高的杂合度,基因型HPA-3aa、HPA-3ab、HPA-3bb频率分别是0.3073、0.4948、0.1979;基因型HPA-15aa、HPA-15ab、HPA-15bb频率分别是0.2708、0.5052、0.2240。结论中山地区血小板献血者HPA-1~6/10/15/21基因与其他地区的同类资料相比显示出地域差异性,建立HPA基因分型资料库有助于解决血小板不配合输注产生同种免疫的问题。Objective To research the genetic polymorphism of HPA 1-6/10/15/21 in platelet donors in Zhongshan area,and establish a gene bank of platelet donors with HPA locus.Methods The HPA 1-6/10/15/21 system genotyping was performed by Real time fluorescent PCR combined with TaqMan probe technology on 192 platelet donors in Zhongshan area,and the genotype frequency and gene frequency were calculated.Results Only HPA-aa genotype was found within HPA-4/10,and no allele HPA-b had been detected.The majority of HPA-1,2,5,6 and 21 genotypes were aa.HPA-3 and HPA-15 showed high heterozygosity,with genotype frequency of 0.3073,0.4948 and 0.1979 for HPA-3aa,HPA-3ab and HPA-3bb,while 0.2708,0.5052 and,0.2240 for HPA-15aa,HPA-15ab and HPA-15bb,respectively.Conclusion The distribution characteristics of HPA 1-6/10/15/21 of platelet donors in Zhongshan shows regional differences compared with similar researches from other regions.The establishment of HPA gene bank is helpful to avoid alloimmunization caused by incompatible platelet transfusion.

关 键 词：实时荧光PCR法 血小板特异性抗原 基因频率 

分 类 号：R331.143[医药卫生—人体生理学] R457.1[医药卫生—基础医学]