全自动加样仪混匀参数对质控品检测结果的影响  

Influence of mixing parameters in STAR automatic sample processing instruments on the detection results of internal controls

作　　者：高静 金建怀 许友山 GAO Jing;JIN Jianhuai;XU Youshan(Wuxi Central Blood Bank,Wuxi 214000,China)

机构地区：[1]无锡市中心血站,江苏无锡214000

出　　处：《中国输血杂志》2023年第3期218-221,共4页Chinese Journal of Blood Transfusion

摘　　要：目的 探讨在酶联免疫吸附试验(ELISA)加样过程中,全自动加样仪对室内质控品进行混匀处理的实验意义。方法 质控品在加样前均进行漩涡震荡混匀,后在相同的检测条件下经STAR全自动加样仪加至同1块酶免板中进行检测,比较质控品在加样针混匀与不混匀情况下S/CO值的差异以及2种情况下质控品检测值的频数分布,受混匀参数影响较大的质控品用同厂家不同批号试剂进行同样的试验并分析结果是否一致。结果 全自动加样仪不对质控品进行混匀处理时,抗-HCV质控品S/CO值明显低于混匀质控品的检测值(P<0.000 1)。部分检测系统所测不混匀抗-TP质控品以及抗-HIV质控品的S/CO值与该项目混匀质控品检测值也有差异(P<0.05)。未经加样针混匀的抗-HCV质控品有部分S/CO值分布在<2的区间。同厂家不同批号试剂分别检测抗-HCV质控品,混匀与不混匀处理的质控品检测值之间均存在差异(部分P<0.05)。结论 尽管加样前质控品均进行漩涡震荡混匀,如果STAR全自动加样仪不设置质控品混匀参数,部分质控品的检测结果依然会受到影响。Objective To explore the significance of blending internal controls by automatic sample processing instruments in the enzyme linked immunosorbent assay(ELISA).Methods The internal controls were vortexed and mixed before the test,and then were added to the same ELISA plate by the STAR automatic sample processing instruments under the same detection conditions.The difference of S/CO value of internal controls with and without sufficient blending via the sampling needle and their frequency distribution were compared.Internal controls that were greatly affected by mixing parameters were submitted to the same test with different batches of reagents from the same manufacturer,and the results were analyzed for consistency.Results The S/CO value of anti-HCV internal controls without blending using adding sample needle was significantly lower than that of quality control samples with sufficient blending(P<0.0001).The S/CO values of unmixed interna controls concerning anti-TP and anti-HIV detection given by some detection systems were also different from the values of mixed internal controls(P<0.05).Some of the S/CO values of the anti-HCV internal controls without mixing were distributed within the interval of less than 2.Different batches of reagents from the same manufacturer were used to detect anti-HCV internal controls,and there were differences in the partial detection values between the mixed and unmixed internal controls(P<0.05).Conclusion Although the internal controls were mixed by vortex shock before the test,the detection results of some internal controls will still be affected when the STAR automatic sample processing instruments does not set the mixing parameters for internal controls.

关 键 词：全自动加样仪 混匀参数 酶联免疫 室内质控品 

分 类 号：R446.1[医药卫生—诊断学]