悬浮红细胞容量范围的确定及其内部控制标准的建立  

Determination of suspended erythrocyte volume range and establishment of its internal control standard

作　　者：兰静 徐华[1] 曹鑫 李佳蔚 丁谨 彭鹏[1] 刘娜 冯娜[1] LAN Jing;XU Hua;CAO Xin;LI Jiawei;DING Jin;PENG Peng;LIU Na;FENG Na(Shaanxi Blood Center,Xian 710000,China)

机构地区：[1]陕西省血液中心,陕西西安710000

出　　处：《中国输血杂志》2023年第3期263-265,共3页Chinese Journal of Blood Transfusion

摘　　要：目的确定悬浮红细胞的容量范围,建立悬浮红细胞容量的内部控制标准。方法根据健康无偿献血者的筛查标准以及《全血及成分血质量要求》的相关要求,计算悬浮红细胞容量的理论值;随机抽取1 U与2 U悬浮红细胞各2410袋进行称量,分别根据“x±2S”与“x±10%”两种规则确定1 U与2 U悬浮红细胞的容量范围;将两种规则确定的容量范围进行比较,制定符合本中心实际的1 U与2 U悬浮红细胞的容量范围。结果1 U与2 U悬浮红细胞的理论容量范围分别为117~160 mL与234~320 mL,抽取的2410袋1 U与2 U悬浮红细胞的实际容量范围分别为142~180 mL与276~393 mL。以“x±2S”为规则制定的1 U与2 U悬浮红细胞的容量范围分别为145~181 mL与298~358 mL,以“x±10%”为规则制定的1 U与2 U悬浮红细胞的容量范围分别为147~179 mL与295~361 mL。质量抽检结果显示,实际容量范围内悬浮红细胞的血细胞比容与血红蛋白含量均符合质量要求。不同地区悬浮红细胞的容量存在不同程度的差异。结论综合实际与近两年悬浮红细胞质量抽检的结果,将163 mL±10%与328 mL±10%分别确定为本中心1 U悬浮红细胞与2 U悬浮红细胞容量的内控标准。各地区可根据各自的实际情况建立准确、可行的悬浮红细胞的容量标准。ObjectiveTo determine the volume rangeendedI erythrocyte and establish its internal control standard.MethodsThe theoretical value of suspended erythrocyte volume was calculated according to the screening criteria of healthy blood donors and Quality Requirements for Whole Blood and Blood Components.A total of 2410 bags of 1 U and 2 U suspended erythrocyte were randomly selected and weighed,and the volume range were formulated by x±2S and x±10%respectively and then compared to determine the volume range in line with the actual situation of our center.Results The theoretical volume range of 1 U and 2 U suspended erythrocyte were 117-160 mL us 234-320 mL,and the actual volume range were 142-180 mL us 276-393 mL.The volume range of 1 U and 2 U suspended erythrocyte formulated by x±2S were 145-181 mL us 298-358 mL,and by x±10%were 147-179 mL vs 295-361 mL.The hematocrit and hemoglobin content of suspended erythrocyte within the actual volume range met the quality requirements.There were fluctuations in the volume of suspended erythrocyte from different regions.Conclusion Based on the actual situation of our center and the sampling results of suspended erythrocytes in recent two years,163 mL±10%and 328 mL+10%were determined as'the internal control standards of 1 U and 2 U suspended erythrocyte,respectively.Blood centers should establish accurate and feasible standard of suspended erythrocyte according to the actual situation.

关 键 词：悬浮红细胞 容量范围 内部控制标准 

分 类 号：R331.141[医药卫生—人体生理学] R457.12[医药卫生—基础医学]