四逆汤配方颗粒和传统饮片汤剂的有效成分比较  

Comparative study on the effective components of Sini decoction formula granules and traditional decoction

作　　者：蒋晨磊 沈哲伦 吕梦泠 刘舟琴 陆红[1] JIANG Chenlei;SHEN Zhelun;LU Mengling;LIU Zhouqin;LU Hong(Zhejiiang Chinese Medical University,Hangzhou,Zhejiiang,310000 P.R.China)

机构地区：[1]浙江中医药大学,浙江杭州310000

出　　处：《华西药学杂志》2023年第2期191-195,共5页West China Journal of Pharmaceutical Sciences

摘　　要：目的比较四逆汤配方颗粒及其传统汤剂中甘草苷、甘草酸、6-姜酚、6-姜烯酚、乌头碱、新乌头碱的含量。方法采用HPLC法检测四逆汤配方颗粒及其传统饮片汤剂中甘草酸、甘草苷、6-姜酚、6-姜烯酚、乌头碱及新乌头碱的含量,分析主要成分在两者中的含量差异,采用C18色谱柱,流动相为乙腈-0.05%磷酸水,梯度洗脱,柱温30℃,检测波长237 nm,流速1.0 mL·min^(-1),进样量10μL。结果四逆汤中甘草苷、甘草酸、新乌头碱、乌头碱、6-姜酚及6-姜烯酚等成分均在测定范围内线性关系良好(r分别为0.9994、0.9997、0.9991、0.9993、0.9992、0.9993),四逆汤传统片汤剂中甘草苷、甘草酸、新乌头碱、乌头碱、6-姜酚、6-姜烯酚的平均回收率分别为98.16%(RSD=1.0%)、100.6%(RSD=l.9%)、98.58%(RSD=1.4%)、98.28%(RSD=1.3%)、100.5%(RSD=1.4%)、99.01%(RSD=1.9%);四逆汤配方颗粒中各成分的平均回收率依次为99.15%(RSD=1.3%)、98.81%(RSD=1.1%)、100.4%(RSD=1.4%)、98.87%(RSD=1.2%)、98.14%(RSD=1.8%)、99.28%(RSD=1.4%)。四逆汤传统汤剂中甘草苷、甘草酸、新乌头碱、乌头碱、6-姜酚、6-姜烯酚质量分数分别为1.627~1.637、1.057~1.075、0.671~0.682、0.540~0.550、0.659~0.678、0.0251~0.0256 mg·g^(-1);配方颗粒中6种成分含量分别为1.866~1.910、2.765~2.826、1.177~1.210、0.989~0.994、0.368~0.373、0.0461~0.0472 mg·g^(-1)。结论与传统汤剂比较,四逆汤配方颗粒中甘草苷、甘草酸、6-姜烯酚、新乌头碱及乌头碱的含量较高,6-姜酚的含量较低。四逆汤配方颗粒较好地保留了饮片中绝大多数的指标成分,为配方颗粒在临床上代替传统饮片使用提供了依据。OBJECTIVE To contrast the contents of liquiritin,glycyrrhizic acid,mesaconitine,aconitine,6-gingerol,6-shogaol and the pharmacodynamic differences in formula granule and traditional decoction of Sini decoction.METHODS The contents of liquiritin,glycyrrhizic acid,mesaconitine,aconitine,6-gingerol,6-shogaol in the extracts obtained from two preparation methods of Sini decoction were determined by HPLC on a C18 column.The mobile phase consisted of acetonitrile-0.05%phosphoric acid in water with gradient elution.Column temperature was set 30℃with a detection wavelength of 237 nm,flow rate of 1.0 mL·min^(-1),and injection volume of 10μL.RESULTS The components of liquiritin,glycyrrhizic acid,mesaconitine,aconitine,6-gingerol,6-shogaol in Sini decoction were all linearly within the range of determination(r were 0.9994,0.9997,0.9991,0.9993,0.9992 and 0.9993).The average recoveries of liquiritin,glycyrrhizic acid,mesaconitine,aconitine,6-gingerol,6-shogaol in traditional decoction of Sini decoction were 98.16%(RSD=1.0%),100.6%(RSD=l.9%),100.5%(RSD=1.4%),99.01%(RSD=1.9%),98.58%(RSD=1.4%)and 98.28%(RSD=1.3%);The average recoveries of each ingredient in formula granules of Sini decoction were 99.15%(RSD=1.3%),98.81%(RSD=1.1%),100.4%(RSD=1.4%),98.87%(RSD=1.2%),98.14%(RSD=1.8%)and 99.28%(RSD=1.4%).The contents of the six constituents in Sini decoction traditional decoction were 1.627-1.637,1.057-1.075,0.671-0.682,0.540-0.550,0.659-0.678,0.0251-0.0256 mg·g^(-1),respectively.And in the formula granule of Sini decoction were 1.866-1.910,2.765-2.826,1.177-1.210,0.989-0.994,0.368-0.373,0.0461-0.0472 mg·g^(-1),respectively.CONCLUSION Compared to the traditional decoction,the contents of liquiritin,glycyrrhizic acid,mesaconitine,aconitine and 6-shogaol in formula granule of Sini decoction had greater levels,but the content of 6-gingerol remained lower.The formula granules of Sini decoction retained most of the index components in decoction pieces,which provided experimental basis for the formula granules to replace traditional

关 键 词：四逆汤 配方颗粒 含量测定 高效液相色谱法 有效成分 传统汤剂 质量标准 比较研究 

分 类 号：R917[医药卫生—药物分析学]