低温致急性全身性冷损伤小鼠动物模型建立  

Establishment of a mouse model of acute systemic cold injury induced by hypothermia

作　　者：田孝野 刘颖 王卓君 陈哲远 程凤 韩晓 丛培芳 史秀云 马瑞珩 金红旭 Tian Xiaoye;Liu Ying;Wang Zhuojun;Chen Zheyuan;Cheng Feng;Han Xiao;Cong Peifang;ShiXiuyun;Ma Ruiheng;Jin Hongxu(Postgraduate Training Base of Jinzhou Medical University at the General Hospital of Northern Theater Command,Shenyang 110016,China;Emergency Medicine Department,General Hospital of Northern Theater Command,Shenyang 110016,China)

机构地区：[1]锦州医科大学北部战区总医院研究生培养基地,沈阳110016 [2]北部战区总医院急诊医学科,沈阳110016

出　　处：《中华急诊医学杂志》2023年第4期521-526,共6页Chinese Journal of Emergency Medicine

基　　金：2021年度军事训练伤防治(21XLS21)。

摘　　要：目的通过环境低温条件,建立急性全身性冷损伤小鼠动物模型。方法C57BL/6小鼠98只,雌雄各半,体质量22~27 g,周龄10周。根据寒冷环境条件小鼠肛温变化将其随机(随机数字法)分成7组(n=14),即A组(38.5±1)℃、B组(35±1)℃、C组(30±1)℃、D组(25±1)℃、E组(20±1)℃、F组(15±1)℃、G组(10±1)℃,其中A组为空白对照组,其余各组为实验组。空白对照组小鼠置于常规环境(205)℃,实验组小鼠置于-20℃低温人工气候箱内,间断测量小鼠肛温(作为核心体温),记录小鼠核心体温分别降至B组、C组、D组、E组、F组、G组所需时间。翻正反射评估小鼠意识状态,观察小鼠行动能力和各器官大体状态,检测小鼠血常规和各器官HE染色。结果随着实验组小鼠设置核心体温越低,则所需时间越长。B、C、D三组小鼠意识状态、行动能力以及各器官大体状态、血常规、各器官HE染色与A组基本一致,未出现急性全身性冷损伤,因此B、C、D组的血常规、器官大体观察、器官HE染色与A组的比较不再展示;与A组相比,E组小鼠开始出现意识状态和行动能力障碍,随着核心体温的降低损伤加重,G组小鼠死亡;与A组相比,E组小鼠血常规(WBC、RBC、HGB、PLT)各指标开始出现降低,单因素方差计算仅WBC变化具有统计学意义(P<0.05);与A组、E组相比,F组小鼠血常规(WBC、RBC、HGB、PLT)各指标进一步降低,单因素方差计算各指标变化均具有统计学意义(P<0.05);大体观察结果显示,与A组相比,E组小鼠肺脏、肝脏、脾脏表面开始出现颜色变暗,与A组、E组相比,F组小鼠肺脏、肝脏、脾脏、肾脏及心脏颜色进一步加深、变暗,可见边缘不齐;各器官HE染色结果显示,与A组相比,E组小鼠开始出现部分肺泡结构破坏以及少量炎症细胞浸润,肝脏中央静脉轻度充血,脾脏红髓与白髓部分见界限不清,与A组、E组相比,F组小鼠的肺脏、肝脏、脾脏、肾脏、心脏及Objective To establish an animal model of acute systemic cold injury in mice.Methods There were 98 C57BL/6 mice,half male and half female,with body weight of 22-27 g and age of 10 weeks.The mice were randomly divided into 7 groups(n=14)according to the changes of anal temperature in cold environment,namely,group A(38.5±1)℃,group B(35±1)℃,group C(30±1)℃,group D(25±1)℃,group E(20±1)℃,group F(15±1)℃,and group G(10±1)℃,among which,group A was the blank control group,and the rest groups were the experimental group.The mice in the blank control group were placed in the normal environment(20±5)℃,and the mice in the experimental group were placed in the low temperature artifi cial climate box at-20℃.The anal temperature of the mice was measured intermittently(as the core temperature),and the time required for the core temperature of the mice to drop to groups B,C,D,E,F and G was recorded.The righting refl ex was used to evaluate the consciousness state,the action ability and the general state of each organ of mice were observed,and the blood routine and HE staining of each organ were detected.Results The lower the core temperature of the experimental group,the longer the time required.The consciousness state,action ability,general state of organs,blood routine,and HE staining of organs in groups B,C,and D were basically the same as those in group A,and there was no acute systemic cold injury.Therefore,the blood routine,general observation of organs,and HE staining of organs in groups B,C,and D were no longer displayed compared with those in group A.Compared with group A,mice in group E began to suffer from disturbance of consciousness and action ability.With the decrease of core body temperature,the damage was aggravated,and mice in group G died.Compared with group A,the indices of blood routine test(WBC,RBC,HGB,PLT)of mice in group E began to decrease,and the univariate variance calculation showed that only WBC changes had statistical signifi cance(P<0.05).Compared with groups A and E,the indic

关 键 词：急性全身性冷损伤 动物模型 意识状态 行动能力 血常规 大体观察 器官HE染色 肛温(核心体温) 

分 类 号：R594.2[医药卫生—内科学] R-332[医药卫生—临床医学]