生姜、干姜、炮姜对胃寒模型大鼠的能量代谢研究  

Study on energy metabolism of fresh ginger,dried ginger and baked ginger on stomach cold model rats

作　　者：杨秀娟 杨志军 陈建平 李硕 田一虹 张淼 谭余庆[2] 隋峰[2] YANG Xiu-juan;YANG Zhi-jun;CHEN Jian-ping;LI Shuo;TIAN Yi-hong;ZHANG Miao;TAN Yu-qing;SUI Feng(College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国临床药理学杂志》2023年第7期989-993,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(82160755,81873024,82173998);甘肃省高等学校创新基金资助项目(2021B-157);甘肃省青年科技基金资助项目(20JR10RA331)。

摘　　要：目的研究生姜、干姜、炮姜对胃寒证模型大鼠的能量代谢,探讨温热药性本质。方法SD大鼠随机分为正常组、模型组(0℃冰水灌胃+10℃泡浴+0.3 mol·L^(-1)冰氢氧化钠刺激)和生姜、干姜、炮姜3个给药组(在模型组基础上分别灌胃给予8.1 g·kg^(-1)生姜、干姜、炮姜),连续给药7 d。用紫外分光光度法测定大鼠肝组织中琥珀酸脱氢酶(SDH)、谷氨酸脱氢酶(GDH)等能量代谢指标,用酶联免疫吸附法测定促肾上腺皮质激素(ACTH)、多巴胺β羟化酶(DβH)、促甲状腺激素(TSH)指标。结果正常组、模型组和干姜、炮姜给药组的Na^(+)-K^(+)-ATP酶含量分别为(0.36±0.06)、(0.29±0.02)、(0.35±0.03)和(0.38±0.09)U·mgprot-1,Ca^(2+)-Mg^(2+)-ATP酶含量分别为(1.07±0.32)、(0.41±0.24)、(0.94±0.27)和(0.87±0.15)U·mgprot-1,GDH含量分别为(5.12±0.80)、(3.07±0.92)、(4.37±1.12)和(4.56±1.38)U·mgprot-1。模型组的上述指标与正常组比较,差异均有统计学意义(P<0.05,P<0.01);干姜、炮姜给药组的上述指标与模型组比较,差异均有统计学意义(P<0.05,P<0.01)。正常组、模型组和生姜、干姜、炮姜给药组的SDH含量分别为(59.69±5.12)、(15.59±7.95)、(24.02±6.74)、(34.08±13.81)和(51.44±11.42)U·mgprot-1,DβH含量分别为(61.96±2.87)、(36.47±1.18)、(45.75±3.07)、(48.38±3.07)和(57.83±1.44)pg·mL^(-1),ACTH含量为(23.51±2.07)、(68.48±5.06)、(61.19±2.54)、(44.51±5.42)和(39.88±5.42)ng·L^(-1),TSH分别为(12.69±1.02)、(5.40±0.40)、(6.90±1.10)、(7.84±1.10)和(11.21±0.51)mU·L^(-1)。模型组的上述指标与正常组比较,差异均有统计学意义(P<0.05,P<0.01);生姜、干姜、炮姜给药组的上述指标与模型组比较,差异均有统计学意义(P<0.05,P<0.01)。结论生姜、干姜、炮姜均可改善寒证模型大鼠的能量代谢,炮姜的改善效果最为显著,温热效应最强,干姜次之,生姜较弱。Objective To study the energy metabolism of fresh ginger,dried ginger and baked ginger in rats with stomach-cold syndrome,and to explore the nature of warming medicinal properties.Methods SD rats were randomly divided into 5 groups:Normal group,model group(gavage with 0℃ice water+10℃cool water bath+stimulation with 0.3 mol·L^(-1)ice sodium hydroxide),and 3 doses of SJ,GJ,and PJ group(8.1 g·kg^(-1)fresh ginger,dried ginger and baked ginger on the basis of the model group)were given intragastric administration continuously for 7 days.The energy metabolism indexes such as succinate dehydrogenase(SDH)and glutamate dehydrogenase(GDH)in rat liver tissue were determined by UV spectrophotometry,and the indexes of adrenocorticotropic hormone(ACTH),dopamineβ-hydroxylase(DβH)and thyroid stimulating hormone(TSH)were determined by enzyme-linked immunosorbent assay.Results The contents of Na^(+)-K^(+)-ATPase in normal group,model group,dried gingergroup and baked ginger group were(0.36±0.06),(0.29±0.02),(0.35±0.03),(0.38±0.09)U·mgprot-1;the contentsof Ca^(2+)-Mg^(2+)-ATPase in these 4 groups were(1.07±0.32),(0.41±0.24),(0.94±0.27),(0.87±0.15)U·mgprot-1;the GDH contents of these four groups were(5.12±0.80),(3.07±0.92),(4.37±1.12),(4.56±1.38)U·mgprot-1,respectively.There were statistically significant differences in the above indexes betweenthe model group and the normal group(P<0.05,P<0.01).Compared with the model group,there were statisticallysignificant differences between the two drug administration groups(P<0.05,P<0.01).The SDH contents of normalgroup,model group,SJ,GJ and PJ group were(59.69±5.12),(15.59±7.95),(24.02±6.74),(34.08±13.81),(51.44±11.42)U·mgprot-1;the DβH contents of these 5 groups were(61.96±2.87),(36.47±1.18),(45.75±3.07),(48.38±3.07),(57.83±1.44)pg·mL^(-1);the ACTH contents of these 5 groupswas(23.51±2.07),(68.48±5.06),(61.19±2.54),(44.51±5.42),(39.88±5.42)ng·L^(-1);the TSHcontents of these 5 groups were(12.69±1.02),(5.40±0.40),(6.90±1.10),(7.84±1.10),(11.21±0.

关 键 词：生姜 干姜 炮姜 胃寒证模型 能量代谢 

分 类 号：R28[医药卫生—中药学]