茶树CsPHT1;3基因特性及其对硒的响应研究  

Study on the Characteristics of CsPHT1;3 and Its Response to Selenium in Tea Plants

作　　者：郭丽娜 郝心愿[2] 王璐[2] 祁蒙 李晓嫚 任恒泽 郑青华 王新超[2] 曾建明 GUO Lina;HAO Xinyuan;WANG Lu;QI Meng;LI Xiaoman;REN Hengze;ZHENG Qinghua;WANG Xinchao;ZENG Jianming(Ankang Research Center of Se-enriched Product,Ankang 726000,China;Tea Research Institute,Chinese Academy of Agricultural Sciences/Key Laboratory of Tea Biology and Resources Utilization Special Economic Animal and Plant Biology and Genetic Breeding,Ministry of Agriculture and Rural Affairs,Hangzhou 310008,China)

机构地区：[1]安康市富硒产品研发中心,陕西安康726000 [2]中国农业科学院茶叶研究所/农业农村部茶树生物学与资源利用特种经济动植物生物学与遗传育种重点实验室,浙江杭州310008

出　　处：《茶叶科学》2023年第2期173-182,共10页Journal of Tea Science

基　　金：中国农业科学院科技创新工程协同任务(CAAS-XTCX20190025-7);中国富硒产业研究院富硒专项“236”计划项目(2019QCY-1.2)。

摘　　要：茶树具有较强的富硒能力,有关磷酸盐转运体参与硒吸收的分子机理研究较少。克隆茶树CsPHT1;3基因,并探究该基因的特性及对硒浓度和价态、pH、时间的响应,以及在不同聚硒茶树品系中的表达情况。基因特性分析表明,CsPHT1;3属于磷酸盐转运蛋白PHT1亚家族成员,定位于质膜且具有PHT1蛋白保守结构域GGDYPLSATIxSE。在不同器官中表达模式分析表明,CsPHT1;3在成熟叶和根中表达量显著高于其他器官。不同浓度和价态硒诱导结果表明,CsPHT1;3在茶树根系中于1 d和7 d显著受低浓度Se^(4+)诱导表达;除处理后3 d外,茶树根系中CsPHT1;3显著受Se^(6+)诱导上调表达且基本不受Se^(6+)浓度影响。不同pH处理茶树结果表明,茶树根系中CsPHT1;3在pH=5时对Se^(4+)响应于24 h达最高;在pH=3时对Se^(4+)响应于48 h达最高;而在pH=7时,对Se^(4+)响应于72 h达最高。硒酸钠处理不同聚硒茶树品系结果表明,CsPHT1;3在不同品系的叶和根中的表达均不响应硒酸钠处理;而亚硒酸钠处理不同聚硒茶树品系结果表明,CsPHT1;3在高聚硒品种叶中显著上调表达。以上研究结果表明,CsPHT1;3可能参与茶树根系对亚硒酸盐的吸收和再分配,其对富硒茶树品系的选育具有重要意义。Selenium(Se)is an essential microelement for human,and Se enriched products are important sources for Se intake in human.Tea plants(Camellia sinensis)have strong selenium enrichment ability.However,there is limited research on the molecular mechanism of phosphate transporters involved in Se absorption.In this study,CsPHT1;3 gene was cloned and its characteristics and responses to Se concentrations,valence,pH,time and expression in various Se-enriched tea resources were investigated.Gene characteristic analysis of CsPHT1;3 shows that CsPHT1;3 is grouped into phosphate transporter PHT1 subfamily and localized in the plasma membrane.The CsPHT1;3 protein contains the conserved domain GGDYPLSATIxSE,which belongs to the PHT1 protein.Expression pattern analysis of CsPHT1;3 in various tissues suggests that the expression levels of CsPHT1;3 in mature leaves and root tissues were significantly higher than those in other tissues.The induction results of different Se concentrations and valence states indicate that CsPHT1;3 was significantly induced by Se^(4+)at 1 d and 7 d after treatments.The expression of CsPHT1;3 in roots was obviously induced by Se^(6+)except for 3 d after treatment but largely unaffected by Se^(6+)concentration.The results of different pH and Se^(4+)treatments show that,at pH5,the highest expression of CsPHT1;3 in tea roots was observed at 24 h.While at pH3,the highest expression of CsPHT1;3 in tea roots was observed at 48 h.Moreover,at pH7,the highest expression of CsPHT1;3 in tea roots was observed at 72 h.The results of sodium selenate treatment on different Se-enriched tea resources indicate that the expression of CsPHT1;3 in the leaves and roots did not respond to sodium selenate treatment.However,the results of sodium selenite treatment on different Se-enriched tea resources suggest that CsPHT1;3 is significantly up-regulated in the leaves of Se-enriched tea resources.The above studies indicated that CsPHT1;3 may participate in the absorption and redistribution of selenite by roots in tea plants,w

关 键 词：茶树 硒吸收 亚硒酸盐 硒 PH 

分 类 号：S517.1[农业科学—作物学] Q786[生物学—分子生物学]