TiO_(2)纳米管阵列负载依布硒缓释体系的优化构建及成骨活性初探  

Optimized construction of TiO_(2) nanotube array loaded with Ebselen sustained-release system and preliminary investigation of its osteogenic activity

作　　者：束长青 姚正华 邱憬[1] SHU Changqing;YAO Zhenghua;QIU Jing(Department of Oral Implantology,Afiliated Stomatological Hospital of Nanjing Medical University,Jiangsu Province Key Laboratory of Oral Diseases,Nanjing 210029,China)

机构地区：[1]南京医科大学附属口腔医院种植科,南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029

出　　处：《口腔医学》2023年第4期294-300,共7页Stomatology

基　　金：国家自然科学基金面上项目(82271003);江苏高校“青蓝工程”中青年学术带头人项目;江苏省科教能力提升工程——江苏省研究型医院(YJXYYJSDW4);江苏省医学创新中心(CXZX202227)。

摘　　要：目的 在钛表面构建二氧化钛纳米管阵列加载依布硒(Ebselen, EB)的缓释体系,探究药物释放动力学及其对成骨细胞行为的影响。方法 通过阳极氧化法在钛基底表面制备出直径120 nm、长2μm的二氧化钛纳米管阵列(TiO_(2)nanotubes, TNT),随后通过冻干法负载不同质量的EB,观察、分析载药后纳米管口的微形貌、表面元素组成以及药物释放特征;将MC3T3-E1成骨细胞接种于不同浓度的EB释出液中,基于微纳米形貌、促成骨性能和药物释放动力学特征,探寻TNT负载EB的最佳加载量。结果 纯钛基底制备形成的TNT在负载EB后可检测出特征性硒元素,当TNT表面负载的EB<5μg/cm^(2)时可完整保留纳米管阵列结构,不同加载量的EB在24 h后均可完全释出,当EB浓度低于10μmol/L时可促进MC3T3-E1的增殖活性和成骨分化,其中,5μmol/L浓度的效果最佳。结论 当TNT表面负载EB<5μg/cm^(2)时纳米管拓扑结构完整,EB浓度在5μmol/L时体外成骨活性最佳。Objective A sustained release system of titanium dioxide nanotubearray loaded with Ebselen(EB)was constructed on a titanium surface to investigate the kinetics of drug release and its effect on osteoblast behaviors.Methods Titanium dioxide nanotubes(TiO_(2)nanotubes,TNT)with length of 2μm and diameter of 120 nm was prepared on titanium substrate by anodic oxidation,and then EB of different mass was loaded by freeze-drying method.The micromorphology,surface element composition and drug release characteristics of the nanotube orifice were observed and analyzed.MC3T3-El osteoblasts were inoculated in different concentrations of EB release solutions,and the optimal loading amount of TNT loaded EB was explored on the basis of micro-nano morphology,osteogenic properties and release kinetic characteristics.Results The selenium element could be detected afer loading EB on TNT prepared on pure titanium substrate.When the EB loaded on TNT was less than 5μg/cm^(2),the TNT array could be completely retained and different amounts of EB could be completely released after 24 h.When the EB concentration was less than 10μmol/L,the proliferation and osteogenic differentiation activities of MC3T3-El were promoted,among which,5μmol/L group showed the best effect.Conclusion When the EB loaded on TNT is below 5μg/cm^(2),the nanotube topological structure remains intact,and the best in vitro osteogenic activity is achieved at the EB concentration of 5μmol/L.

关 键 词：TiO_(2)纳米管 依布硒 药物输送 成骨细胞 

分 类 号：R783.1[医药卫生—口腔医学]