紧密连接相关蛋白在变应性鼻炎小鼠模型中的表达研究  

Expression of tight junction proteins in mice with allergic rhinitis

作　　者：郑少川 瞿申红[2] 张少杰[2] 钟自玲 张力行 程熹乔 莫丽萍 吴迪 Zheng Shaochuan;Qu Shenhong;Zhang Shaojie;Zhong Ziling;Zhang Lixing;Cheng Xiqiao;Mo Liping;Wu Di(Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Department of Otolaryngology,Head and Neck,The People’s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530000,Guangxi,China;Department of Pathology,The People’s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530000,Guangxi,China)

机构地区：[1]右江民族医学院,广西百色533000 [2]广西壮族自治区人民医院耳鼻咽喉头颈科,广西南宁530000 [3]广西壮族自治区人民医院病理科,广西南宁530000

出　　处：《右江民族医学院学报》2023年第2期237-241,共5页Journal of Youjiang Medical University for Nationalities

基　　金：国家自然科学基金项目(81960186)。

摘　　要：目的研究紧密连接相关蛋白在变应性鼻炎(allergic rhinitis,AR)小鼠模型中的表达变化,并初步探究其与羧肽酶A3之间的联系。方法使用卵清蛋白致敏小鼠建立AR的小鼠模型,在最后1次鼻腔激发后观察小鼠行为学变化,收集小鼠血清和鼻黏膜组织,通过常规病理、免疫组织化学和实时荧光定量PCR技术来评估小鼠鼻黏膜羧肽酶A3及紧密连接相关蛋白的表达变化。结果AR组小鼠表现出明显的打喷嚏和抓鼻症状;与Control组相比,AR组小鼠羧肽酶A3表达显著上升(P<0.001),而Claudin-1、Claudin-4和JAM-A表达较Control组降低(P<0.05)。结论羧肽酶A3可能通过鼻黏膜紧密连接的破坏参与AR的发病。Objective To study the expression of tight junction proteins in mice with allergic rhinitis(AR),and to explore the relationship between tight junction proteins and carboxypeptidase A3.Methods Ovalbumin sensitized mice were used to establish the AR mouse models,and behavioral changes of mice were observed after the last nasal stimulation.Serum and nasal mucosa of mice were collected.The expression changes of tight junction proteins and carboxypeptidase A3 in nasal mucosa of mice were evaluated by routine pathology,immunohistochemistry and real-time quantitative PCR.Results The mice in AR group showed obvious symptoms of sneezing and scratching nose.Compared with the control group,the expression of carboxypeptidase A3 in AR group significantly increased(P<0.001),while the expressions of Claudin-1,Claudin-4 and JAM-A decreased(P<0.05).Conclusion Carboxypeptidase A3 may be involved in the pathogenesis of AR through the destruction of tight junction proteins of nasal mucosa.

关 键 词：鼻炎 变应性 上皮屏障 紧密连接部 羧肽酶A3 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]