基于二氧杂环丁烷骨架的化学发光探针发展和应用研究  

Development and Application of Dioxetane⁃based Chemiluminescent Probes

作　　者：傅安辰 毛彦佳 王宏博 曹志娟[1] Anchen Fu;Yanjia Mao;Hongbo Wang;Zhijuan Cao(School of Pharmacy,Fudan University,Shanghai 201203,China;China State Institute of Pharmaceutical Industry,Incubation Center for S&T Achievements,Shanghai 201203,China)

机构地区：[1]复旦大学药学院,上海201203 [2]中国医药工业研究总院科技成果转化中心,上海201203

出　　处：《化学进展》2023年第2期189-205,共17页Progress in Chemistry

基　　金：上海市科学技术委员会农业领域项目(No.21N31900500);上海市卫生健康委员会项目(No.202140016);复旦大学附属闵行医院基础医学青年人才项目(No.2021MHJC10)资助。

摘　　要：光学分析法具有无损、实时和空间分辨能力的特点,是研究疾病发生、发展和诊疗的一种重要技术手段,包括荧光、生物发光和化学发光(CL)。近年来,以金刚烷稳定的二氧杂环丁烷为骨架的化学发光探针(AD⁃CL)获得关注。该探针不但具有无需激发光源,可避免光漂白、光毒性,灵敏度较高的CL法传统优势;且具有检测体系简单,无需氧化剂的参与,可在生理条件下发光等特点。最近,经过进一步的结构优化与改造,AD⁃CL骨架探针在生理条件下的发光性能大幅提升,在物质检测、光学成像等领域中的应用越来越广泛。本文综述了AD⁃CL探针的发光原理以及最新的研究进展,主要分为两部分:AD⁃CL探针的优化与改造策略以及AD⁃CL探针在不同物质检测的应用。Optical analysis is non⁃destructive,real⁃time with a specific spatial resolution,which has been developed as an essential technology to study the occurrence,development,diagnosis,and treatment of diseases.It contains fluorescent(FL),bioluminescent(BL)and chemiluminescent(CL)methods.Among them,CL probes with an adamantane⁃dioxetane chemiluminescence(AD⁃CL)scaffold attracted much attention.Recently,significant improvement on these probes has been achieved with the elimination of external light source,low phototoxicity,high sensitivity,and a facile system without additional reagents,such as oxidants.Until now,the CL probes were further developed with special modifications and new synthesis routes based on the AD⁃CL scaffold,realizing the detection and optical imaging of various biomolecules in living systems with enhanced properties.Herein,the recent research progress on AD⁃CL probes has been reviewed.The review is divided into two parts.The first part will mainly introduce the molecular modification strategy of AD⁃CL probes and the second part will focus on their application in several cases.

关 键 词：二氧杂环丁烷 化学发光 探针 分子设计 光学成像 

分 类 号：O644[理学—物理化学]