大豆磷转运蛋白GmPHO1;7基因克隆、表达分析与功能  

Cloning,Expression Analysis and Function of Soybean Phosphorus Transporter GmPHO1;7 Gene

作　　者：刘丽薇 付禹 于人杰 何旭 杨雪[1] 杨美英[1] LIU Liwei;FU Yu;YU Renjie;HE Xu;YANG Xue;YANG Meiying(College of Life Sciences,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学生命科学学院,长春130118

出　　处：《吉林农业大学学报》2023年第1期14-21,共8页Journal of Jilin Agricultural University

基　　金：吉林省自然科学基金项目(20170101160)。

摘　　要：以“长农26”为材料,克隆获得全长为2295 bp的核苷酸序列,编码765个氨基酸,命名为GmPHO1;7。生物信息学分析表明,GmPHO1;7的氨基酸序列具有完整的SPX与EXS结构域,该基因启动子区含有低磷相关元件P1BS,可能定位是在细胞膜上的跨膜蛋白,属于磷转运蛋白PHO1家族。酵母功能互补试验证明Gm⁃PHO1;7具有转运磷的能力。基因表达分析发现,在正常磷(0.5 mmol/L KH2PO4)和低磷(0.01 mmol/L KH2PO4)条件下,GmPHO1;7的表达受低磷诱导并且主要在根部发挥功能。GUS染色与含磷量测定结果证明,GmPHO1;7基因可以在大豆发根中正确表达,且可以增加发状根的含磷量。Using Changnong 26 as the material,this study cloned a 2295 bp nucleotide sequence encoding 765 amino acids,named GmPHO1;7.Bioinformatics analysis showed that the amino acid sequence of GmPHO1;7 has complete SPX and EXS domains.The promoter region of this gene con⁃tains the low-phosphorus-related element P1BS,which may be a transmembrane protein located on the cell membrane and belongs to the PHO1 family of phosphorus transporters.Yeast complementation experiments proved that GmPHO1;7 has the ability to transport phosphorus.Gene expression analysis under normal phosphorus(0.5 mmol/L KH2PO4)and low phosphorus(0.01 mmol/L KH2PO4)conditions revealed that the expression of GmPHO1;7 is induced by low phosphorus and functions mainly in the roots.GUS staining and determination of phosphorus content proved that GmPHO1;7 gene can be cor⁃rectly expressed in soybean hairy roots and can increase the phosphorus content of hairy roots.

关 键 词：大豆 磷转运蛋白 GmPHO1 7基因 发状根 

分 类 号：S565.1[农业科学—作物学]