褐煤提质废水生物处理系统启动期菌群结构变化  

Dynamic Changes of Microbial Community Structure in the Start-up Period of the Biological Treatment System for Lignite Upgrading Wastewater

作　　者：邓欢 陆海[2] 张小雨 刘志生 闫博佼 任贺 DENG Huan;LU Hai;ZHANG Xiaoyu;LIU Zhisheng;YAN Bojiao;REN He(College of Civil Engineering and Architecture,Changchun Sci-Tech University,Changchun 130600,China;Key Laboratory of Songliao Aquatic Environment,Ministry of Education,Jilin Ji-anzhu University,Changchun 130118,China;Changchun Institute of Urban Planning and De-sign,Changchun 130022,China)

机构地区：[1]长春科技学院建筑工程学院,长春130600 [2]吉林建筑大学松辽流域水环境教育部重点实验室,长春130118 [3]长春市城乡规划设计研究院,长春130022

出　　处：《吉林农业大学学报》2023年第1期83-93,共11页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(51808254)。

摘　　要：利用Illumina MiSeq测序平台,定量分析褐煤提质废水生物接触氧化反应器生物膜培养驯化过程中的微生物种群变化规律,旨在为处理系统的优化运行提供科学依据。试验依据褐煤提质废水的进水比例分为30%、60%以及100%3个阶段。结果表明:经驯化,生物接触氧化反应器对褐煤提质废水的COD去除率为85.55%~88.21%,满足后续深度处理系统的进水要求;随着进水褐煤提质废水比例的升高,生物膜微生物种群丰富度降低,种群多样性降低;随着启动过程的进行,进水酚类等芳香族物质含量升高,种群结构产生了动态响应,Flavobacteria、Aquaspirillum等菌属由于不适应水质,逐渐被淘汰,而可降解多种有机物尤其是芳烃类的Hydrogenophaga等菌属逐渐成为优势菌属。为通过工程技术手段创造更适合Hydrogenophaga等功能菌属生境,以强化菌群功能、提高褐煤提质废水处理效果提供理论依据。Illumina MiSeq sequencing platform was used to quantitatively analyze the dynamic changes of the microbial population in the biofilm cultivation and domestication process of lignite up⁃grading wastewater in biological contact oxidation reactor,aiming to provide a scientific basis for the optimal operation of the treatment system.The experiment was divided into three stages:30%,60%and 100%respectively according to the influent proportion of lignite upgrading wastewater.The re⁃sults showed that after acclimation,the COD removal rate of lignite extracted wastewater in the bio⁃logical contact oxidation reactor reached 85.55%-88.21%,which met the influent water require⁃ments of the subsequent in-depth treatment system.With the increase in the proportion of lignite up⁃grading wastewater extracted from influent lignite,the microbial population richness and diversity de⁃creased.With the start-up process,the content of phenols and other aromatic substances in the influ⁃ent increased,and the population structure produced a dynamic response:Flavobacteria,Aquaspiril⁃lum and other bacteria were gradually eliminated because of their inadaptability for the water quality.The bacteria capable of degrading a variety of organic substances,especially aromatic hydrocarbons,such as Hydrogenophaga,gradually became the dominant bacteria.Therefore,scientific support was provided to aid in creating more suitable habitats for functional bacteria such as Hydrogenophaga through engineering techniques,to enhance the function of flora and improve the treatment effect of lignite extraction wastewater.

关 键 词：褐煤提质废水 生物接触氧化 微生物种群结构 

分 类 号：X703[环境科学与工程—环境工程]