免疫胶体金法与间接免疫荧光法检测对下呼吸道感染患儿肺炎支原体感染的诊断价值分析  

Clinical value of immune colloidal gold technique and indirect immunofluorescence assay in diagnosis of mycoplasma pneumonia infection in children with lower respiratory tract infection

作　　者：曲奎尧 张珊 谢春艳 董彦茂 张传仓 Qu Kuiyao;Zhang Shan;Xie Chunyan;Dong Yanmao;Zhang Chuancang(Pediatric Department,No.971 Naval Hospital,Qingdao 266071,China)

机构地区：[1]海军第九七一医院儿科,山东青岛266071

出　　处：《海军医学杂志》2023年第3期270-274,共5页Journal of Navy Medicine

摘　　要：目的评价免疫胶体金法(immune colloidal gold technique,GICT)与间接免疫荧光法(indirect immunofluores⁃cence assay,IFA)对下呼吸道感染患儿肺炎支原体(mycoplasma pneumoniae,MP)感染诊断的临床价值。方法收集2019年在海军第九七一医院利用GICT与IFA行MP⁃IgM抗体检测的134例下呼吸道感染患儿的临床资料,比较2种检测方法对MP感染的阳性率、临床符合率,以及2种检测方法分别在不同年龄组、不同病程组之间的差异。结果GICT的MP检测阳性例数为62例(46.3%),IFA的MP检测阳性例数为99例(73.9%),2种方法比较差异有统计学意义(P<0.01)。GICT对MP感染的临床符合率为54.5%,IFA的临床符合率为80.6%,差异有统计学意义(P<0.01)。2种检测方法分别在不同年龄组对MP感染阳性率比较差异无统计学意义(P>0.05)。按照在不同病程分组,4~7 d组和>7 d组GICT对MP检测阳性率分别为34.2%、58.6%,差异有统计学意义(P=0.005);4~7 d组和>7 d组IFA对MP检测阳性率分别为65.8%、84.5%,差异有统计学意义(P=0.015)。在>7 d组的58例中,2种检测方法均阳性有30例,该30例患儿均符合MP感染临床诊断,阳性的临床符合率为100%。结论2种检测方法对MP感染的诊断均有一定的临床价值,IFA对MP感染的检测阳性率及临床符合率均更高;病程>7 d的MP检测阳性率更高;GICA与IFA联合检测均阳性的结果与MP感染的临床诊断一致。病程4~7 d仍需结合临床或联合其他实验室诊断方法进行判断。Objective To evaluate the clinical value of immune colloidal gold technique(GICT)and indirect immunofluores⁃cence assay(IFA)in diagnosis of mycoplasma pneumonia(MP)infection in children with lower respiratory tract infection.Methods The clinical data of 134 child patients with lower respiratory tract infection who underwent MP⁃IgM antibody detection by using GICT and IFA in the hospital in 2019 were collected for study.All children had the GICT on the 1st day and IFA on the 2nd day to detect the MP⁃IgM antibody.The positive rate and clinical compliance rate of MP infection were compared between the 2 detection methods,and also differences of the two detection methods used in different age groups and different disease course groups were also compared between the groups.Results The numbers of MP positive infection cases were respectively 62(46.3%)by GICT and 99(73.9%)by IFA.There was statistical significance in MP positive detection rates,when comparisons were made between the 2 detection methods(P<0.01).The clinical compliance rate of MP infection by GICT was 54.5%and by IFA was 80.6%,with statistical significance(P<0.01).There was no statistical significance in the positive detection rates of MP infection,when the 2 detection methods were used in different age groups(P>0.05).The positive detection rates of MP infection by GICT in different disease course groups(4⁃7⁃day group and>7⁃day group)were respectively 34.2%and 58.6%,with statistical significance(P=0.005).The positive detection rates of MP infection by IFA were 65.8%and 84.5%respectively,also with statistical significance(P=0.015).In the 58 cases of the>7⁃day group,there were 30 positive cases by both detection methods.All the 30 child patients were in line with the clinical diagnosis of my⁃coplasma infection,with the positive compliance rate of 100%.Conclusion The 2 detection methods are of certain clinical value for the diagnosis of MP infection,with the IFA method having higher detection rate of MP infection and higher compliance rate.The

关 键 词：免疫胶体金法 间接免疫荧光法 肺炎支原体 儿童 

分 类 号：R446.61[医药卫生—诊断学]