中国中医科学院西苑医院2019-2021年尿路感染病原菌分布与耐药监测  

Distribution and drug resistance monitoring of pathogenic bacteria in urine culture of Xiyuan Hospital, China Academy of Chinese Medical Sciences from 2019 to 2021

作　　者：王忠[1] 王庆勇 高庆和[1] 邵魁卿[1] 陈豪特 王雄[1] 周艳艳 高瞻[1] WANG Zhong;WANG Qingyong;GAO Qinghe;SHAO Kuiqing;CHEN Haote;WANG Xiong;ZHOU Yanyan;GAO Zhan(Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing100091,China)

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《中国医药导报》2023年第10期142-144,148,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(82104880);中国中医科学院科技创新工程项目(CI2021A01908);中国中医科学院基本科研业务费自主选题项目(Z0546)。

摘　　要：目的探讨中国中医科学院西苑医院尿路感染病原菌分布情况,并分析不同病原菌的耐药性。方法收集2019年1月至2021年12月中国中医科学院西苑医院尿路感染患者的尿培养标本,采用基质辅助激光解析电离飞行时间质谱仪进行菌株鉴定,BD Phoenix-100全自动细菌鉴定药敏系统测定最低抑菌浓度。WHONET 5.6统计药敏结果数据,SPSS 25.0统计学软件进行数据分析。结果获得尿路感染病原菌1191株,革兰氏阴性菌以大肠埃希菌和肺炎克雷伯菌为主;革兰氏阳性菌以粪肠球菌和无乳链球菌为主。产超广谱β-内酰胺酶(ESBLs)和非产ESBLs的大肠埃希菌对庆大霉素的敏感率差异无统计学意义(P>0.05);产ESBLs菌株对其余常用药物的敏感率均低于非产ESBLs菌株(P<0.05)。结论革兰氏阴性菌仍是尿路感染的主要病原菌,大肠埃希菌和肺炎克雷伯菌中ESBLs和耐药菌株检出率较高,粪肠球菌对常用药物也存在耐药,应加强医院细菌耐药监测,合理使用抗生素。Objective To investigate the distribution of pathogenic bacteria of urinary tract infection in Xiyuan Hospital,China Academy of Chinese Medical Sciences and analyze the drug resistance of different pathogenic bacteria.Methods Urine culture samples were collected from patients with urinary tract infection in Xiyuan Hospital,China Academy of Chinese Medical Sciences from January 2019 to December 2021.The isolated strains were identified by MALDI-TOF MS,and the BD PheniX-100 automatic bacterial identification drug sensitivity system was used to determine the minimum inhibitory concentration.WHONET 5.6 was used for drug susceptibility data statistics,and SPSS 25.0 was used for data analysis.Results A total of 1191 strains of urinary tract infection pathogens were obtained.The main Gram-negative bacteria were Escherichia coli and Klebsiella pneumoniae.The main Gram-positive bacteria were Enterococcus faecalis and Streptococcus agalactiae.There was no significant difference in the sensitivity rate of extended-spectrumβ-lactamases(ESBLs)-producing and non-ESBLs-producing Escherichia coli to gentamicin(P>0.05).The sensitivity rate of ESBL-producing Escherichia coli to other commonly used drugs was lower than that of non-ESBL-producing Escherichia coli(P<0.05).Conclusion The detection rate of ESBLs and drug-resistant strains in Escherichia coli and Klebsiella pneumoniae is high.Enterococcus faecalis is also resistant to commonly used drugs.We should strengthen the monitoring of bacterial resistance in hospitals and use antibiotics rationally.

关 键 词：中段尿培养 病原菌 耐药性 中医院 

分 类 号：R691.3[医药卫生—泌尿科学]