抗-HCV确证试验中阳性及不确定结果带型分析  

Band type analysis of positive and uncertain results in anti-HCV confirmatory test

作　　者：袁宗智 邓建平[1] 张斌[1] 李群[1] 李科 王红[1] YUAN Zongzhi;DENG Jianping;ZHANG Bin;LI Qun;LI Ke;WANG Hong(Zigong Center for Disease Control and Prevention,Sichuan 643000,China)

机构地区：[1]自贡市疾病预防控制中心,四川643000

出　　处：《医学动物防制》2023年第3期219-222,共4页Journal of Medical Pest Control

基　　金：传染病预防控制国家重点实验室开放课题(2016SKLID309);四川省卫生计划和生育委员会科研课题(150259)。

摘　　要：目的分析本实验室以免疫印迹法(western blotting,WB)测定的丙型肝炎病毒(Hepatitis C virus,HCV)抗体阳性、不确定结果带型及不确定者抗体变化情况。方法将筛查试验酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA)为阳性的176例标本进行确证试验(WB)并分析。结果176例阳性标本经确证试验结果为阳性156例,不确定8例,阴性12例。156例WB阳性带型结果中全带型43例,出现率为27.6%,4条带型(核抗原、NS3-1、NS3-2、NS5)31例,出现率为19.9%,4条带型(核抗原、NS3-1、NS3-2、NS4)19例,出现率为12.2%,3条带型(核抗原、NS3-2、NS5)、2条带型(核抗原、NS3-1)和仅有核抗原1条带型的各11例,出现率均为7.1%。8例不确定者其WB带型结果中NS3-1、NS4(±)的不确定带型组3例(出现率为37.5%),其余带型组合均为不同的1例存在[核抗原(+);核抗原(+)、NS4(±);核抗原(+)、NS5(±);核抗原(+)、NS3-1(±)、NS4(±);NS3-1(±)、NS3-2(±)、NS4(±)];从带型分布看NS3-2、NS4(±)的结果为5例(出现率为62.5%),其次为核抗原(+)4例(出现率为50.0%),NS5(±)为1例(出现率为12.5%)。结论2015—2020年四川省自贡市抗-HCV阳性标本WB带型以核抗原为主,NS3-1次之。抗-HCV不确定标本以NS3-2、NS4带型为主,出现此带型时应加强随访以避免漏检。Objective To analyze the positive antibody of the Hepatitis C virus(HCV)tested by western blotting(WB)in our laboratory,and the uncertain result type and uncertain antibody changes.Method Confirmatory WB assay was performed and the result was analyzed of 176 samples that were positive by enzyme linked immunosorbent assay(ELISA).Results The experimental results of 176 positive specimens were confirmed as positive in 156 cases,uncertain in 8 cases and negative in 12 cases.Among the 156 cases of WB positive band type,43 cases were full band type,with an occurrence rate of 27.6%;31 cases were 4 band types(nuclear antigen,NS3-1,NS3-2,NS5),with an occurrence rate of 19.9%;19 cases were 4 band types(nuclear antigen,NS3-1,NS3-2,NS4),with an occurrence rate of 12.2%.There were 11 cases with 3 banding types(nuclear antigen,NS3-2,NS5),2 banding types(nuclear antigen,NS3-1)and only nuclear antigen 1 banding type,and the occurrence rate was 7.1%.Among the 8 uncertain WB band type results,there were 3 cases(the occurrence rate was 37.5%)of NS3-1 and NS4(±)uncertain band type group,among which 1 case with other band type combinations were different had nuclear antigen(+);nuclear antigen(+);NS4(±);nuclear antigen(+);NS5(±);nuclear antigen(+),NS3-1(±),NS4(±);NS3-1(±),NS3-2(±),NS4(±).According to the band type distribution,the results of NS3-2 and NS4(±)were 5 cases(the occurrence rate was 62.5%),followed by 4 cases of nuclear antigen(+)(the occurrence rate was 50.0%),and 1 case of NS5(±)(the occurrence rate was 12.5%).Conclusion From 2015 to 2020,the WB band type of anti-HCV positive specimens in Zigong City,Sichuan Province is mainly nuclear antigen,followed by NS3-1.The anti-HCV uncertain specimens are mainly NS3-2 and NS4 band types,which should be followed up to avoid missed detection.

关 键 词：免疫印迹法 HCV 不确定结果 带型 分析 

分 类 号：R512.63[医药卫生—内科学]