黄连素和黄芩苷不同配伍对肠道细菌体外生长的影响  

Effects of different combinations of berberine and baicalin on the growth of intestinal bacteria in vitro

作　　者：江文琳 段志伟 陈梦婕 刘艺敏 刘敬昊 余冬 严小军[1] 吉燕华 曾治君[1] JIANG Wen-lin;DUAN Zhi-wei;CHEN Meng-jie;LIU Yi-min;LIU Jing-hao;YU Dong;YAN Xiao-jun;JI Yan-hua;ZENG Zhi-jun(Research Center for Differentiation and Development of Traditional Chinese Medicine(TCM)Basic Theory,Jiangxi Key Laboratory of TCM Etiopathogenesis,Jiangxi University of Chinese Medicine,Nanchang,Jiangxi 330004,China;不详)

机构地区：[1]江西中医药大学中医基础理论分化发展研究中心,江西省中医病因学重点实验室,江西南昌330004

出　　处：《中国微生态学杂志》2023年第3期269-274,共6页Chinese Journal of Microecology

基　　金：国家自然科学基金地区项目(81760787);江西省中医药中青年骨干人才项目(赣中医药科教字〔2021〕2号);江西中医药大学校级科技创新团队发展计划(CXTD22005,CXTD22007);江西中医药大学校级研究生创新专项(JZYC22S76)。

摘　　要：目的研究不同配伍的黄连素和黄芩苷在模拟肠道厌氧环境下对肠道内有害菌、条件致病菌和益生菌体外生长的影响。方法采用微量肉汤稀释法测定黄连素和黄芩苷不同配伍对肠道内金黄色葡萄球菌、沙门菌、肠炎链球菌、大肠埃希菌、乳杆菌及嗜黏蛋白阿克曼菌体外生长24 h后吸光度的影响。结果对于金黄色葡萄球菌,抑制作用最强的是黄连素∶黄芩苷=10∶1(组合六)125μg/mL(t=–39.350,P<0.001),抑制率为99.91%;对于沙门菌,抑制作用最强的是黄连素∶黄芩苷=100∶1(组合七)250μg/mL(t=–73.788,P<0.001),抑制率为80.47%;对于肠炎链球菌,抑制作用最强的是黄连素∶黄芩苷=1∶0(组合一)250μg/mL(t=–57.549,P<0.001);对于大肠埃希菌,抑制作用最强的是黄连素∶黄芩苷=1∶0(组合一)125μg/mL(t=–29.085,P<0.001);对于乳杆菌,增殖作用最强的是黄连素∶黄芩苷=100∶1(组合七)31.25μg/mL(t=76.280,P<0.001);对于嗜黏蛋白阿克曼菌,增殖作用最强的是黄连素∶黄芩苷=100∶1(组合七)62.5μg/mL(t=8.357,P<0.001)。结论黄连素∶黄芩苷=10∶1(组合六)、黄连素∶黄芩苷=100∶1(组合七)对有害菌的抑制作用更强,黄连素∶黄芩苷=100∶1(组合七)对益生菌的增殖作用更强,而黄连素和黄芩苷联合使用对条件致病菌的抑制作用较弱。Objective To study the effects of different combinations of berberine and baicalin on the growth of harmful bacteria,opportunistic pathogens and probiotics in vitro under simulated anaerobic environment.Methods The microdilution method was used to determine the changes of absorbance values of berberine and baicalin on the intestinal Staphylococcus aureus,Salmonella,Streptococcus enteritidis,Escherichia coli,Lactobacillus and Akkermansia muciniphila after 24 hours of growth.Results For Staphylococcus aureus,the combination with highest inhibitory effect was berberine∶baicalin=10∶1(combination six)125μg/mL(t=–39.350,P<0.001),with the inhibition rate being 99.91%;for Salmonella,it was berberine∶baicalin=100∶1(combination seven)250μg/mL(t=–73.788,P<0.001),with the inhibition rate being 80.47%;for Streptococcus enteritidis,it was berberine∶baicalin=1∶0(combination one)250μg/mL(t=−57.549,P<0.001);for Escherichia coli,berberine∶baicalin=1∶0(combination one)125μg/mL(t=–29.085,P<0.001);for Lactobacillus,berberine∶baicalin=100∶1(combination seven)31.25μg/mL(t=76.280,P<0.001);and for Akkermansia muciniphila,it was berberine∶baicalin=100∶1(combination seven)62.5μg/mL(t=8.357,P<0.001).Conclusion The combinations of Berberine∶baicalin=10∶1(combination six)and berberine∶baicalin=100∶1(combination seven)can promote the inhibition against harmful bacteria,and the combination of berberine∶baicalin=100∶1(combination seven)can promote the proliferation of probiotics,while the combined use of berberine and baicalin has no obvious promoting effect on the inhibition to neutral bacteria.

关 键 词：黄连素 黄芩苷 肠道细菌 抑制作用 增殖作用 

分 类 号：R285[医药卫生—中药学]