电针对应激性心肌病小鼠心功能及感觉、运动皮层局部场电位的影响  

Effects of electroacupuncture on cardiac function and local field potential of sensory and motor cortices in mice with stress cardiomyopathy

作　　者：吴昊晟 苏航 朱超 王文慧 吴生兵[2,3] 崔帅[2,4] 周美启[4,5] WU Hao-sheng;SU Hang;ZHU Chao;WANG Wen-hui;WU Sheng-bing;CUI Shuai;ZHOU Mei-qi(Graduate School,Anhui University of CM,Hefei 230012,China;Institute of Acupuncture and Meridian,Anhui University of CM;Key Laboratory of Xin'an Medical Science,Ministry of Education;School of Acupuncture and Moxibustion,Anhui University of CM,Hefei 230012;Bozhou Institute of TCM,Anhui Academy of Chinese Medical Sciences,Bozhou 236800)

机构地区：[1]安徽中医药大学研究生院,合肥230012 [2]安徽中医药大学针灸经络研究所 [3]新安医学教育部重点实验室 [4]安徽中医药大学针灸学院,合肥230012 [5]安徽省中医药科学院亳州中医药研究所,亳州236800

出　　处：《中国针灸》2023年第4期379-389,共11页Chinese Acupuncture & Moxibustion

基　　金：国家重点研发计划项目:2022YFC3500500、2022YFC3500502;安徽省中医药领军人才建设项目:ZYYLJRC201911。

摘　　要：目的:观察电针对应激性心肌病(SC)小鼠心功能及感觉、运动皮层局部场电位(LFP)的影响,探讨电针改善SC的可能机制.方法:将27只雌性C57BL/6小鼠随机分为空白组、模型组和电针组,每组9只.模型组、电针组小鼠予腹腔注射异丙肾上腺素(ISO)14 d制备SC模型.造模同时,电针组予电针"内关""神门",疏密波,频率2 Hz/15 Hz,每次15 min,每日1次,干预14 d.干预后,观察各组小鼠旷场实验5 min内总运动距离、跨格次数和中央格跨格次数;采用超声心动图检测各组小鼠左心室功能[舒张末期左心室内径(LVIDd)、收缩末期左心室内径(LVIDs)、舒张末期左心室容积(LVEDV)、收缩末期左心室容积(LVESV)、射血分数(EF)和缩短分数(FS)];观察各组小鼠心电图ST段振幅和PR间期数据;HE染色法观察各组小鼠心肌组织形态;ELISA法检测各组小鼠血清皮质醇(CORT)、心肌肌钙蛋白T(cTnT)及脑钠肽(BNP)含量;应用Plexon多通道信号采集处理系统记录各组小鼠感觉、运动皮层LFP变化.结果:与空白组比较,模型组小鼠旷场实验总运动距离、跨格次数、中央格跨格次数减少(P<0.05);LVIDd、LVIDs、LVEDV、LVESV升高(P<0.05),EF、FS降低(P<0.05);心电图ST段振幅升高、PR间期延长(P<0.05);心肌纤维排列紊乱,间质水肿,炎性细胞浸润;血清CORT、cTnT、BNP含量升高(P<0.05);感觉皮层delta、theta、alpha、beta频段所占比率升高(P<0.05),theta、beta频段最大能量谱升高(P<0.05),delta、theta、alpha、beta、gamma频段功率谱密度(PSD)升高(P<0.05);运动皮层delta、theta、alpha、beta频段所占比率均升高(P<0.05),delta、theta、alpha、beta、gamma频段最大能量谱和PSD升高(P<0.05).与模型组比较,电针组小鼠旷场实验总运动距离、跨格次数、中央格跨格次数增加(P<0.05);LVIDd、LVIDs、LVEDV、LVESV降低(P<0.05),EF、FS升高(P<0.05);ST段振幅下降、PR间期缩短(P<0.05);心肌纤维损伤及炎性细胞浸润减轻;血清CORT�Obetive To ohserve the efeets of electroacupuneture(EA)on cardiac funetion and loeal field potential(LFP)in sensory and motor cortices in mice with stress cardiomyopathy(SC),and to explore the possible mechanism of EA in improving SC.Methods Twenty-seven female C57BL/6 mice were randomized into a blank group,a model group and an EA group,9 mice in each group.In the model group and the EA group,SC model was established by continuous intraperitoneal injection of isoproterenol(ISO)for 14 days.At the same time of modeling,EA was applied at"Neiguan"(PC 6)and"Shenmen"(HT 7)in the EA group,with disperse-dense wave,in frequency of 2 Hz/15 Hz,15 min each time,once a day for 14 days.After intervention,the total movement distance,the number of crossing grid and the number of crossing central grid of open field test within 5 minutes were observed;the left ventricular function indexes left ventricular diameter of end-diastole[LVIDd],left ventricular diameter of end-systole[LVIDs],left ventricular volume of end-diastole[LVEDV],left ventricular volume of end-systole[LVESV],ejection fraction[EF]and fraction shortening[FS]were detected by echocardiography;the changes in ST-segment amplitude and PR interval of electrocardiogram were observed;the morphology of myocardial tissue was observed by HE staining;the serum levels of cortisol(CORT),cardiac troponin T(cTnT)and brain natriuretic peptide(BNP)were detected by ELISA;the changes of LFP in sensory and motor cortices were recorded by Plexon multi-channel acquisition system.Results Compared with the blank group,in the model group,the total movement distance,the number of crossing grid and the number of crossing central grid of open field test were decreased(P<0.05):LVIDd,LVIDs,LVEDV and LVESV were increased(P<0.05),EF and FS were decreased(P<0.05);ST-segment amplitude was increased(P<0.05)and PR interval was prolonged(P<0.05);irregular myocardial fiber arrangement,interstitial edema and inflammatory cell infiltration were observed;the serum levels of CORT,cTnT and BNP were increased

关 键 词：应激性心肌病 电针 心功能 感觉皮层 运动皮层 场电位 

分 类 号：R245.97[医药卫生—针灸推拿学]