1株伪狂犬病病毒的基因变异及其对小鼠的致病性分析  

Genetic Variation and Pathogenicity of a Pseudorabies Virus Variant Strain in Mice

作　　者：倪征[1] 叶伟成[1] 陈柳[1] 云涛[1] 华炯钢[1] 朱寅初 张存[1] NI Zheng;YE Weicheng;CHEN Liu;YUN Tao;HUA Jionggang;ZHU Yinchu;ZHANG Cun(Institute of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省农业科学院畜牧兽医研究所,杭州310021

出　　处：《畜牧兽医学报》2023年第4期1766-1770,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：浙江省基础公益研究计划(LGN21C180002)。

摘　　要：本研究旨在了解我国猪伪狂犬病病毒(pseudorabies virus,PRV)流行毒株的分子遗传变异及毒力特点。对1株分离自1个接种了Bartha-K61疫苗的规模猪场的PRV ZJ株进行了主要糖蛋白基因序列(gB、gC和gE)的测定分析及对6周龄BALB/c小鼠的致病性研究。遗传变异分析显示,该毒株与我国2012年后的流行毒株高度同源,具有变异毒株基因特征,但gE基因的510位氨基酸出现了不同于变异株的独特变化S^(510) G。ZJ株对小鼠具有较高的致病性,攻毒小鼠呈现典型的神经症状,以100 LD_(50)感染小鼠,第5天死亡率达100%。本研究为进一步探索该病毒的毒株变异特点与致病机理奠定基础。This study aimed to understand the molecular genetic variation and virulence characteristics of porcine pseudorabies virus(PRV)in China.The main glycoprotein gene sequences(gB,gC and gE)of PRV ZJ strain isolated from a pig farm inoculated with Bartha-K61 vaccine were determined.and its pathogenicity to 6-week-old mice was studied.Phylogenetic analysis results showed that this strain was highly homologous to epidemic strains in China after 2012,and had the genetic characteristics of the mutant strain.However,there was a unique change of S^(510) G in the amino acid position 510 of gE gene,which was different from the mutant strain.ZJ strain was highly pathogenic to BALB/c mice,and the challenged mice showed typical neurological symptoms.The mortality rate reached 100%on day 5 after the mice were infected with 100 LD_(50).This study laid a foundation for further exploration of the variation characteristics and pathogenesis of this virus.

关 键 词：伪狂犬病病毒 变异株 遗传变异 致病性 

分 类 号：S852.659.1[农业科学—基础兽医学]